碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 105 / Phaeodactylum tricornutum is one of two diatoms with genome sequenced. In the Diatom EST database, over 30,000 expressed sequence tags (ESTs) from Phaeodactylum tricornutum have already been identified. Therefore, Phaeodactylum tricornutum serves as a good model organism for understanding cellular response of primary producers in the ocean when facing environmental changes. The effects of ocean acidification on diatoms were addressed in a few studies before; however, systematic and comprehensive proteomics analysis is still obscure. Additionally, few studies were taken under a well-controlled environment which involves an automatic CO2 adjustment system to maintain a stable pH condition in diatom culture. The 15N-labeling SILAC method has been previously examined in Phaeodactylum tricornutum culture. There are no obvious biological differences in cell morphology, growth rate, and proteomics profile between 15N and 14N culture conditions. Thus, the 15N-labeling SILAC is a suitable method for quantitative proteomics analysis. Our preliminary result shows that levels of photosynthesis-related proteins were significantly altered in acidified environment. More detailed analyses are still undergoing.
| Identifer | oai:union.ndltd.org:TW/105NTOU5613013 |
| Date | January 2017 |
| Creators | Jiang, Tai-Yi, 江泰頤 |
| Contributors | Hsu, Pang-Hung, 許邦弘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 45 |
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