碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 105 / A high expression of UV-damaged-DNA binding activity in zebrafish embryos was repeatedly detected in previous studies and two 30 to 35-kDa polypeptides homologous to the 150-kDa zebrafish vitellogenin 1 (zfVg1) have been identified as components of the embryonic UV-binding activity. Because of the inhibition of nucleotide excision repair (NER) capacity in zebrafish extracts by a monoclonal anti-zfVg1 antibody found in recent studies, low-molecular-weight zfVg1-like polypeptides may also participate in NER of UV-damaged DNA. The goal of this study was to examine if oxidative stress induced by paraquat (PQ) and hydrogen peroxide (HP) would disturb NER and the production of zfVg 1-like molecules in zebrafish embryos. A transcription-based in vitro DNA repair assay showed differential effects of PQ and HP at 30 to 200 μM on NER capacity in zebrafish embryos. NER activities monitored by the up-regulation of UV-suppressed marker cDNA transcription were lowered to 19.8% of control in embryos exposed to 200 μM HP, while these activities remained at 90.3% of control in PQ-exposed embryos. In contrast, gene activities encoding NER-associated DNA damage recognition proteins UV-DDB2 and XPC were not affected by PQ or HP at 200 μM according to real-time RT-PCR. Compared to the extracts of control embryos, higher levels of 35 to 55 kDa polypeptides recognized by the anti-Vg1 monoclonal antibody were repeatedly detected in the extracts of PQ and HP-exposed embryos and the highest level of the 35-kDa zfVg1-like factor was present in the extracts of embryos treated with 200 μM HP, suggesting a link between the inhibition of NER in these embryos and an abnormal pattern of zfVg1 cleavage induced by HP.
| Identifer | oai:union.ndltd.org:TW/105NTOU5613024 |
| Date | January 2017 |
| Creators | Lai, Po-Ling, 賴柏伶 |
| Contributors | Hsu,Todd, 許濤 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 35 |
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