碩士 / 國立臺北教育大學 / 自然科學教育學系 / 105 / Streptococcus spp. and Iridovirus often cause a large number of deaths from cultured fish, and epidemiology is widely distributed in southern Taiwan. Streptococcal infection was treated with Tilapia (Tilapia nilotica x Tilapia aurea) and Perch (Lates calcarifer). Sick fish will have hemorrhagic sepsis, mainly swimming is not normal, body color black, prominent eye, mouth and gill cover bleeding phenomenon. In this study, we should explore the pathogen, take the diseased fish spleen for bacterial isolation and purification. 16S rDNA and 16S-23S rDNA PCR were carried out and the product was cloned and sequenced. The results were compared with those in GenBank. 16S rDNA is 1500 bp, 16S-23S rDNA of Tilapia is 600 bp, 16S-23S rDNA of Perch is 800 bp, it can be seen from the Tilapia isolated for the Streptococcus agalactiae; Perch isolated for the Streptococcus iniae. Iridovirus infection often causes a large number of deaths of grouper (Epinephelus spp.). In the province, the sick fish have no obvious signs of appearance, but their breathing is reduced, the feeding rate is reduced and the activity is reduced. Sick fish gill tablet can be seen giant cells, and internal organs splenomegaly, sliced observation in the gills and spleen can also be seen huge cells exist. In the spleen organs can be seen in the basophilic nuclear inclusion body. In this study, we should quickly explore the pathogen, take the internal organs of diseased fish for tissue sections and H&E staining. Take the spleen tissue 0.3 g, the tissue grinding and made of emulsion. Centrifuge, take the supernatant, quantitative 0.1 (μg/μl). In the GenBank to obtain a pair of primers, respectively CY15n-F, CY15n-R, PCR amplifycation, available 1,339 bp, you can confirm the infection iridovirus. Therefore, effective application PCR technology to detect fish farming, can increase the income of farmers.
| Identifer | oai:union.ndltd.org:TW/105NTPT0147010 |
| Date | January 2017 |
| Creators | TSAI,MENG-CHUN, 蔡孟珺 |
| Contributors | Lu,Chow-Chin, 盧秀琴 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 40 |
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