博士 / 國立臺灣大學 / 食品科技研究所 / 105 / Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structural characteristics of the polysaccharides. The polysaccharides had Mw of 3.45 × 105 Da. The major sugars were L-fucose, L-arabinose, D-galactose, D-xylose, and D-galacturonic acid in the molar ratio of 6.4, 15.6, 28.7, 14.8, and 24.3% with a small amount of D-glucose, D-mannose, L-rhamnose, and D-glucuronic acid. Results indicated that the bioactive polysaccharides consisted of a (1,4)-Galp and (1,4)-GalAp back bone; (1,2)-Xyl and (1,2)-Rha might also comprise backbone or constitute side chain; linkage (1,5)-Ara and terminal fucosyl residues were also involved in the polysaccharides. Regarding bioactivity, removal of the terminal L-fucosyl residues reduced the TNF-α cytokine stimulating activity of the polysaccharides in a RAW264.7 macrophage cell-line test, whereas NF-kapaB and TLR4 affected the polysaccharide-induced TNF-α production. Quanternary selecting precipitation as a strategy to rapid enriches fucose-containing acidic polysaccharide, deoxyl sugar contain from 4.2% upgrade to 15.6% by one step separated.
| Identifer | oai:union.ndltd.org:TW/105NTU05252019 |
| Date | January 2017 |
| Creators | Hsueh-Ting Wang, 王雪婷 |
| Contributors | Ting-Jang Lu, 呂廷璋 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 106 |
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