碩士 / 國立臺灣大學 / 工程科學及海洋工程學研究所 / 105 / The aim of this study was attempted to find out new compounds instead of traditional biocide to decrease the toxicity of coating and prevent the heavy metal pollutions. Deteced the bactericidal activity of compound by microbial experimental, determined whether bacteria attached, biofilm formation and bactericidal activity by electrochemical experimental. Finally, Optical microsocope, SEM and CLSM were used to certifiy previous experiments.
The first part was microbial experiments, extracted 16S rNDA of sample which collected form Danshui, Taiwan and compared difference of sequence with database to determine the species of bacteria. After that, different concentrations of thymol were added into culture broth to find the bactericidal activity at different concentrations for six strains, draw the growth curves and determine the MIC(MIC，Minimum inhibitory concentration) of six strains. The results revealed that thymol had great bactericidal activity and its MIC was about 125μg/L.
The second part was electrochemical experiments, decteded the attachment of bacteria and bactericidal activity of thymol by open circuit potential (OCP) and Electrochemical impedance spectrum (EIS). The results of OCP In open-circuit potential, presence and attachment of bacteria causing potential oscillation, while the test environment added thymol and sterile electrolyte is more stably；In EIS analysis, we found that the trend of data is adverse in presence of bacteria and sterile environment.
According to these informations, the bactericidal activity and attachment of bacteria could be judge by electrochemical testing. OM, SEM and CLSM were used to observe the surface morphology and biofiom formation.
| Identifer | oai:union.ndltd.org:TW/105NTU05345060 |
| Date | January 2017 |
| Creators | Jie-Yu Chang, 張介宇 |
| Contributors | 李岳聯 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 102 |
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