碩士 / 國立臺灣大學 / 漁業科學研究所 / 105 / This study aims to promote artificial rearing and breeding techniques of Oplegnathus punctatus via record of embryonic ontogeny, larval and juvenile development and establishment of a feeding program for the species, and then assigned toexperiment II to know the effect of dietary protein level on the growth and body composition of O. punctatus juvenile.In the first trial, used the injection of hCG 1000 IU/kg BW and LHRH - α 20μg / kg treatment to induce spawning of female O. punctatus and were artificially fertilized from April to May 2017. The fertilized pelagic eggs are transparent with diameter of 1.03 ± 0.04 mm and single oil globule with diameter of 0.23 ± 0.01 mm. The eggs hatched in 30 hours after fertilization at 23 ± 1 ℃. The newly-hatched larvae are 1.96 ± 0.01 mm in standard length. From 3 to 33 dph the larvae can be fed with rotifers and copepods enriched with Nannochloropsis oculata and Tetraselmis chui, bigger copepods and nauplii can be fed as larvae grows up. Metamorphosis occurred in 28 dph and artificial feed was mixed with artemia to train the larvae to accept. After 44 dph, the larvae developed into juvenile stage with 26 mm in standard length. In the second trial, O. punctatus juvenile with initial weight 16 g fed on six diets with same energy and lipid level (7 %) but different dietary protein levels of 35, 40, 45, 50 %, 55 % and 60 % for eight weeks. The result showed the weight gain were between 227.79 ± 46.24 % - 477.47 ± 24.42 %, specific growth rate (SGR) were between 2.10 ± 0.26 - 3.13 ± 0.08, feed conversion ratio (FCR) were between 1.05 ± 0.13 - 1.67 ± 0.49. Fish fed diet with 55 % protein has the highest weight gain (477.47 ± 24.42 %), and was significant higher than diets with 35 - 50 % protein (p< 0.05). Based on the weight gain using broken-line model analysis showed the optimal dietary protein level for O. punctatus juvenile with initial weight of 16 g cultured under 29 ± 1 ℃ is 57.58 %. Weight gain, SGR were between 2.10 ± 0.26 - 3.13 ± 0.08 and were improved with dietary protein increased from 35 % to 55 %. SGR in diets with 55 % protein was significant higher than diets with 35 - 50 % protein (p< 0.05). FCR were between 1.05 ± 0.13 - 1.67 ± 0.49 and improved with dietary protein increased. Fish fed 55 % has lowest FCR (1.05 ± 0.13) and significant lower than groups fed 35 - 45 % protein diets. Coefficient factor (CF) was not significantly increased by dietary protein level among 40 - 60 % groups (p> 0.05). Hepatosomatic index (HSI) and viscerosomatic index (VSI) was not generally affected with dietary protein. Crude protein in muscle increased with increasing dietary protein. Crude lipid and Ash in muscle was not significantly affected with dietary protein among 40 - 60 % groups.
| Identifer | oai:union.ndltd.org:TW/105NTU05451017 |
| Date | January 2017 |
| Creators | Tsung-Han Lin, 林宗翰 |
| Contributors | Wen-Liang Liao, Fan-Hua Nan, 廖文亮, 冉繁華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 126 |
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