博士 / 國立臺灣大學 / 醫學工程學研究所 / 105 / Biomaterials are often added to autologous fat grafts both as supporting matrices for the grafted adipocytes and as cell carrier for adipose-derived stem cells (ADSCs). In this study, a new materials were synthesized by linking developmentally essential ECM and laminin to alginate(Alg). The production of new material was followed by an in vivo study, which used an autologous fat graft model to test the laminin-alginate biomaterial, adipocytes, and ADSCs in immune-competent rats.
In this study, we transplanted different combinations of shredded autologous adipose tissue [designated “A” for adipose tissue]), laminin-alginate beads [designated “B” for bead], and ADSCs [designated “C” for cell]) into the backs of 15 Sprague-Dawley rats. Group A received only adipocytes, Group B received only laminin-alginate beads, Group AB received adipocytes mixed with laminin-alginate beads, Group BC received laminin-alginate beads encapsulating ADSCs, and Group ABC received adipocytes and laminin-alginate beads containing ADSCs. Seven-tesla magnetic resonance imaging was used to evaluate the rats at the 1st, 6th, and 12th weeks after transplantation. At the 12th week, the rats were sacrificed and the implanted materials were retrieved for gross examination and histological evaluation.
The results based on MRI, gross evaluation, and histological data all showed that implants in Group ABC had better resorption of the biomaterial, improved survival of the grafted adipocytes, and adipogenic differentiation of ADSCs. Volume retention of grafts in Group ABC (89%) was also significantly greater than those in Group A (58%) (p<0.01). Our findings support that the combination of shredded adipose tissue with ADSCs in laminin-alginate beads provided the best overall outcome.
| Identifer | oai:union.ndltd.org:TW/105NTU05530010 |
| Date | January 2017 |
| Creators | Yo-Shen Chen, 陳右昇 |
| Contributors | Feng-Huei Lin, 林峰輝 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 43 |
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