碩士 / 國立臺灣科技大學 / 材料科學與工程系 / 105 / In this study, we synthesized biocompatible vaccine nanocarrier hybrid polysaccride Fucoidan. Our research including two parts:
In the first part, Gelaitn was hybrided with Fucoidan by chemical crosslinking method, a mean size was observed by DLS & SEM images, which located between 200~400nm nanoparticle, crosslinking structure has been comfirmed by IR spectrum. A high loading efficiency (45%) and high stability carrier (only 10% of drug were loss during 2 weeks) was prepared, which was measured by UV-vis spectrum. Furthermore, we observed drug loading image by CLSM after stability test.
In the second part, we utilized Click chemistry to combine Fucoidan and Polycaprolactone, which created Fucoidan-graft-Polycaprolactone amphiphilic polymer(FD@PCL), we comfirmed azide and alkynyl group IR signal at 2100cm-1, and then we identified Triazole ring signal around 8.0ppm of 1H NMR, under FD: PCL=1:50 synthesis condition.
Moreover, we produced high stability, high drug loading efficiency carrier transforming from THF to water condition, surprisingly we discovered that size distribution approximately located at 200nm and increased while drug loading, eventually demonstrated 86% encapsulation efficiency, which maintain below 10% loss during one month, measuring by UV-vis spectrum. At last, we observed nanoparticles containing green fluorescence images by CLSM.
Keywords:Gelatin、Biocompatible polymers、Fucoidan、vaccine carrier、 Chemical crosslink、Amphiphilic polymer、Polycaprolactone.
| Identifer | oai:union.ndltd.org:TW/105NTUS5566034 |
| Date | January 2017 |
| Creators | Kun-Lin Hsieh, 謝坤霖 |
| Contributors | Shuenn-kung Su, 蘇舜恭 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 124 |
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