碩士 / 慈濟科技大學 / 放射醫學科學研究所 / 105 / Abstract
The purpose of this study was to investigate the healing potential and possible mechanism of enteromorpha prolifera extraction (EE) and enteromorpha prolifera polysaccharides (EP) on the wounds treated with UV irradiation. In this study, human foreskin fibroblast cells (HS-68) were used to simulate wound healing. The experimental conditions were divided into six groups, including control group, enteromorpha prolifera extraction treatment group, enteromorpha prolifera polysaccharides treatment group, UV treatment group (66 mJ/cm2), UV plus enteromorpha prolifera extraction treatment group and UV plus enteromorpha prolifera polysaccharide treatment group.
This research has revealed that both EE and EP could promote the rate of cell migration. It was convinced that the expression of IL-6 could accelerate the healing of wound. The results showed that the EE had significant antioxidant capacity compared with the EP, and the EE could avoid UV irradiation induced G2/M phase cell cycle arrest in HS-68. From the results, it was concluded that the EE and EP could accelerate the healing of wound by stimulating the secretion of IL-6 from skin fibroblasts, and induce the cell activation and enhance the cell migration rate. The pathways may be related to MAPK / ERK phosphorylation in the PTKs family.
| Identifer | oai:union.ndltd.org:TW/105TCCN0605005 |
| Date | January 2017 |
| Creators | Chen, Cheng-Li, 陳政莉 |
| Contributors | Liu, Wei-Chung, 劉威忠 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 47 |
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