碩士 / 慈濟技術學院 / 放射醫學科學研究所 / 103 / Abstract
Many of inflammatory cytokines or cancers have also been demonstrated to induce osteoclast activity and differentiation. In clinical treatments, irradiation or bisphosphonate are utilized to reduce the bone pain in patients who suffer from bone lesion. Alginate oligosaccharide (AOS) extracted from seaweed not only has the anti-oxidant abilities, but also inhibits the inflammatory. However, the effects of AOS on osteoclatogenesis have not been well-established. The aim of this study we have investigated the effects of AOS or combined with radiation on the differentiation of osteoclast induced by receptor activator of NF-κB ligand (RANKL) and possible related mechanisms.
In this study, monocytes (RAW264.7 cells) were used to evaluate the effects of osteoclastogenesis. Three groups including the control, AOS treatment, and a combinative treatment of AOS and radiation (5 Gy) were divided in the experiment. Results show that AOS could inhibit the CD51, a mark of pre-osteoclast, expression induced by RANKL; besides, AOS could down-regulate the nuclear factor kappa-B protein and tartrate resistant acid phosphatase (TRAP) production and decrease the osteoclast differentiation. Moreover, AOS could enhance the effect of radiation- induced inhibition in osteoclast differentiation.
Our results suggest that AOS can decrease the osteoclast number possibly via inhibiting the monocytes to decrease the production of NF-κB protion, although probably not the only one.
| Identifer | oai:union.ndltd.org:TW/105TCCN0605023 |
| Date | January 2015 |
| Creators | Kao, Sheng-Yu, 高聖育 |
| Contributors | Liu, Wei-Chung, 劉威忠 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 49 |
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