碩士 / 國立雲林科技大學 / 化學工程與材料工程系 / 105 / Microalgae have great potential in cosmeceutical, biomedical, and green energy conversion applications. Developing an effective methodology enabling fast and high-throughput analysis of the physicochemical properties of microalgae in aqueous solution is still of great-demand. In this study, we develop a new microfluidic method that combines a sheath flow and the cell dielectrophoresis in a designed serpentine microchannel to continuously separate microalgae with different lipid contents. Due to the different extents of non-uniform electric field between theserpentine microchannel and microalgae with different cell conductivities, microalgae with different lipid contents experience different magnitudes of dielectrophoretic force. The parametric effects of the applied potentials in the driving and sheath flow-controlled electrodes on the dielectrophoretic separation of microalgae with lower and higher lipid contents are investigated systematically. It is found that a mixture of microalgae with lower and higher lipid contents can be continuously separated when a 250V DC is applied in the electrokinetic driving electrode and a 300~330V DC is applied in the sheath flow-controlled electrode when the surfactant is absent.
| Identifer | oai:union.ndltd.org:TW/105YUNT0661012 |
| Date | January 2017 |
| Creators | WENG,TSUNG-CHIEH, 翁琮傑 |
| Contributors | YEH, LI-HSIEN, 葉禮賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 116 |
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