碩士 / 元智大學 / 化學工程與材料科學學系 / 105 / The purpose of this study is to fabricate a three-layer sandwich-like structure combined two electrospun membranes with porous sponge-like structure in between as a repair patch for tendon tissue engineering. The electrospun membranes were prepared with the solutions of poly(3-hydroxybutyrate) (PHB) and polycaprolactone or grapheme oxide (GO), the porous sponge-like structure was fabricated with silk fibroin. Aligned fibrous membranes could be fabricated by electrospinning with oriented structure to enhance the mechanical strength and guide the alignment of cells. Via X-ray diffractometer, overall crystallinity of PHB blends demonstrated an decreasing trend. The mechanical properties of PHB were optimized by blending with PCL or adding GO as nanofillers. The cell viability of 3T3 fibroblasts and tenocytes on the electrospun membranes were evaluated by WST-1 assay in 7-dat cultures. On the other hand, the diameter of membrane fibers could be adjusted by using various concentrations of polymer solution. It was observed that cell proliferation was regulated by the changes in the fiber diameter, as the higher the fiber diameter the better the cell viability was obtained.
On the other hand, the crystallization of silk fibroin could be induced by aqueous ethanol (EtOH) and isopropanol (IPA). Afterwards, silk fibroin was lyophilized to form a porous sponge-like structure scaffold with pore size ranging from 5 to 10 μm with controllable thickness. The sponge-like porous structure in the scaffold could provide a microenvironment for cell proliferation and infiltration.
| Identifer | oai:union.ndltd.org:TW/105YZU05063050 |
| Date | January 2017 |
| Creators | Guan-An Lai, 賴冠安 |
| Contributors | Yi-Ming Sun, 孫一明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 119 |
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