碩士 / 高雄醫學大學 / 生物醫學暨環境生物學系碩士班 / 106 / Copper nanoparticles (CuNP) are widely applied in piezoelectric devices and fuel cells. Large-scale production and consumption of CuNP-containing products could lead to discharge of CuNP into aquatic ecosystems. In addition, increasing atmospheric carbon dioxide (CO2) resulted in warming and acidification of aquatic ecosystem, further affecting on physicochemical properties of CuNP and its accumulated by aquatic organisms. However, previous studies investigated the effects of single environmental factor on the CuNP bioaccumulation. The effects of multiple factors on bioaccumulation of CuNP are still unknown. The purpose of this study is to assess whether warming and CO2 induced acidification synergistically increase the bioaccumulation of CuNP in tilapia (Oreochromis niloticus). Tilapia were exposed to waterborne 25 nm CuNP for 7-day uptake and transferred to clean water for 7-day in following different environmental scenarios, combined with acidification groups (pHs 7.5, 6.5 and 5.5) and warming groups (26, 28 and 30°C), and to analyze accumulation of gill and muscle. Results indicated that warming factor did not increase accumulation of the CuNP concentration in gill and muscle for uptake phase. At 7-day depuration phase of warming experiment, the accumulated concentration of high warming group (30°C/pH 7.5) in gill was 3.68 ± 0.89 µg g-1, and significantly higher than control (26°C/pH 7.5; 1.00 ± 0.46 µg g-1, p < 0.05). Moreover, the concentration of muscle in high warming group (1.63 ± 0.20 µg g-1) was also significantly higher than control (0.61 ± 0.04 µg g-1) at 1-day depuration phase (p<0.05). It revealed that warming would slow-down the copper depuration in gill and muscle. Results of acidification effects demonstrated that the accumulated concentration of high acidification group (26°C/pH 5.5) in gill was 8.75 ± 0.83 µg g-1, which significantly higher than control (5.10 ± 0.68 µg g-1) at 7-day uptake phase (p < 0.01). However, concentration of gill in all acidification groups during depuration phase [low acidification (26°C/pH 6.5): 4.09 ± 0.32 µg g-1; high acidification (26°C/pH 5.5): 2.89 ± 0.14 µg g-1] were significantly higher than control (1.01 ± 0.46 µg g-1, p < 0.001). The acidification factor did not increase the CuNP concentration in muscle for uptake phase, whereas the concentrations of high acidification group at 1- and 7-days of depuration phase were 1.45 ± 0.12 µg g-1 and 1.39 ± 0.16 µg g-1, respectively, and significantly higher than control (0.6 ± 0.04 µg g-1, p < 0.001). Therefore, acidification would increase the copper uptake in gill, and slow down the copper depuration in gill and muscle. Under multiple factors scenarios, the low warming and acidification (28°C/pH 6.5) significantly affected the bioaccumulation of CuNP in uptake phase (p < 0.01), but there was no effect at 7 days. The multiple factors would also significantly slow down the copper depuration in gill (p < 0.05). Moreover, there was no significantly difference among CuNP concentration of control and multiple factors groups in muscle at 1- and 7-days of uptake phase. In depuration phase, multiple factors significantly affected the accumulated concentrations at 7-day (p < 0.01), but did not at 1-day. In conclusion, this study suggested that acidification mainly increases the bioaccumulation of CuNP in tilapia, and multiple factors synergistically retard the depuration of CuNP in gill and muscle for depuration phase.
| Identifer | oai:union.ndltd.org:TW/106KMC05114003 |
| Date | January 2018 |
| Creators | Jin-Liang Kuo, 郭晉良 |
| Contributors | Wei-Yu Chen, 陳韋妤 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 69 |
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