碩士 / 明志科技大學 / 化學工程系碩士班 / 107 / In the modern life nowadays, food and health are important issues of concern to society. In this perspective, the research to improve health, protect against disease and ageing has begun several investigations and investment studies throughout the world. Zeaxanthin is one such natural pigment emphasized for its critical role in the prevention of age-related macular degeneration (AMD), the leading cause of blindness. Zeaxanthin is chemically synthesized or purified from bacteria; however, increasing demand requires development of alternative sources such as heterologous biosynthesis by marine bacteria. However, the commercialization of Zeaxanthin bioprocess is largely dependent on the price of feed stocks. Renewable cellulose appears to be an appealing feedstock. To address this issue, in this research, the cellulose hydrolysate of sugarcane bagasse will be used as carbon sources and Mesoflavibater zeaxanthinifaciens isolasated from seaweed to produce Zeaxanthin. In this study, growth and production of zeaxanthin by Mesoflavibater zeaxanthinifaciens were studied using glucose as a carbon source in the basal medium. Mesoflavibacter zeaxanthinifaciens is motile by gliding and produces the carotenoid pigment zeaxanthin. Here, we report the molecular identification of zeaxanthin producing sponge-associated bacteria and the effect of glucose on zeaxanthin production from a marine bacteria. After pretreatment sugarcane bagasse to produce glucose, the highest yields of glucose (8.5 g/L) were obtained in the reaction mixture containing 1% Cellulast enzyme and sugarcane bagasse treated with KOH 1.5% and 1% H2O2. After all bacteria were cultivated in the basal medium, Mesoflavibacter zeaxanthinifaciens was found to produce the highest zeaxanthin at 100 mg/L. Its optimal condition for zeaxanthin production was found with extraction efficiency 70% ethanol solvent. Therefore, the zeaxanthin production via marine bacteria has a potential to produce natural zeaxanthin for the food, feed, pharmaceutical, and cosmetic industries. The best growth was supported by sucrose, but glucose yielded similar carotenoid concentrations, both asparagine and glutamine stimulated growth and pigment formation, Carotenoid production and glucose consumption increased as a function of asparagine concentration. Finally, the aim of this research will be to develop and producing Zeaxanthin using raw materials.
| Identifer | oai:union.ndltd.org:TW/106MIT00062012 |
| Date | January 2018 |
| Creators | DO THI NGOC TRAM, 杜氏玉貞 |
| Contributors | CHIEN, LIANG -JUNG, 簡良榮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 46 |
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