碩士 / 國立中興大學 / 生命科學系所 / 106 / Environmental temperature is a major abiotic factor that affects different modulations in many aspects of the organisms. When facing low temperature, energy requirement for compensation was needed. Mitochondria is a well-known energy support organelle in the cell, and its morphological dynamic constantly changes. The status of mitochondria is modulated by the dynamic of morphological changes when cell is under stimulation. Changes in mitochondrial morphology and biogenesis can increase the energy supply for physiological changes in fish. In this study, it was hypothesized that different low-temperatures induced changes in mitochondrial dynamics and biogenesis in livers of freshwater (FW)- and seawater (SW)-acclimated Indian medaka (Oryzias dancena). In the results of investigating mitochondrial dynamics, Dynamin-related protein 1 (Drp-1) protein significantly decreased at 7th day in FW group at 15 °C, while increased at 2nd hour and restored to originally normal level at 12th hour in the SW group at 18 °C and 15 °C. The results of mtDNA contents together with the voltage-dependent anion-selective channel 1 (VDAC1) expressions that were not affected in all conditions revealed that mitochondria might change its dynamic but not the content. Low temperature resulted in different patterns of cytochrome c oxidase (COX) subunit expression. COX 1 was not affected in the FW group at 18°C and 15°C, while the SW group increased COX1 expression at the 2nd hour, restored to originally normal level at 12th hour at 18 °C, and decreased at 24th hour at 15 °C. However, COX4 protein abundance increased in the all conditions. The expressions of citrate synthase (CS) were significantly increased except that in the FW group at 18 °C. In summary, when facing low temperature, in the FW group, mitochondrial fragmentation was reduced and increased the expression of CS in the matrix to supply energy at 15 °C. In the SW group, however, fragmentation of mitochondria might delay the occurrence of mitochondrial biogenesis.
| Identifer | oai:union.ndltd.org:TW/106NCHU5105035 |
| Date | January 2018 |
| Creators | Yu-Hsin Chen, 陳雨鑫 |
| Contributors | 李宗翰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 63 |
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