碩士 / 國立中興大學 / 園藝學系所 / 106 / In the present study, we investigated the flowering physiology and pollen viability in different type of begonia species and applied as the parents to cross with Taiwan endemic species, such as Begonia fenicis and B. chitoensis. To promote breeding efficiency and reduce breeding schedule, the embryo rescue technique was used to create the relative with Taiwan begonia species and novel characteristic of begonia hybrids. In addition, we applied the colchicine treatment to restore the good traits of commercial species without pollen fertility for future breeding work. By poliploidization, we expected to enhance the adptability of crops under the unfavorable enviroments and to creat gerplasm resources with diverse polidy.
Taiwan endemic begonia species were grown in the Taichung Wufeng district, and anthesis period were as follows: Begonia fenicis blossomed from February to August, B. chitoensis blossomed form December to May of next year, Rex begonia blossomed from November to February, April and May of next year, Cane-like begonia and semperflorens begonia blossomed all year-round. Tuberous begonia blossomed just limited from December to March of next year. In Dewitte medium, the optimal temperature and sucrose concentration for pollen germination of begonia species were 25-30℃ and 10% respectively by incubation in dark environment for one hour.
Using Begonia chitoensis and B. fenicis as pollen donor, all combinations were obtained progenies successfully in the interspecific hybridization experiment. However, when B. chitoensis was used as maternal donor, crossing with B. ''Kagayaki'' can obtain the offspring successfully. When B. fenicis was utilized as maternal donor, crossing with B. ''KinKan'', B. ''Poseidon'', B. ''Kagayaki'' and B. ''Honoho-no-mai'' the partial combination can only partially obtained hybrid offspring. It seems to exhibit unilateral incompatibility in the reciprocal cross between our researches. Most characteristics of the interspecific hybrids were shown in intermediate type to their parents. Progenies crossed by Rex begonia and B. chitoensis had the increase anthesis period and show more tiller of plant type. Progenies crossed by cane-like begonia and B. chitoensis had the reduced plant height and enhanced tiller. Some individual plant had male flower precocious phenomenon. Progenies crossed by B. semperflorens and B. chitoensis had the similar plant type with pollen donor. Progenies crossed by begonia tuberous and B. chitoensis showed were no tuber geophyte, but increased the tiller of plant and period of anthesis. Progenies crossed by Rex begonia and B. fenicis had the similar plant type as maternal donor, but the growth potential were weak. Progenies crossed by cane-like begonia and B. fenicis had inconspicuous internodes, shorter plants, and more tiller. Partially individual plants had male flowers precocious phenomenal. Progenies crossed by B. semperflorens and B. fenicis had similar leaf texture with male parent. Progenies crossed by begonia tuberous and B. fenicis exhibit no tuber geophytel, weak growth potential, and mosaic leaves. The relative DNA content of progenies was found in intermediate value between their parents by flow cytometry. Using RAPD, all the hybrid progenies obtained specific bands from their parents which confirmed the real hybrids.
The result of different concentration clochicin treatment in vivo and in vitro, demonstrated that the Begonia semperflorens ''Big®-Bronze leaf-Rose'' had the best muntant efficiency in 10 g L-1 colchicine solution with cotton, and totally 7 polyploidy mutants were obtained. For in vitro treatment of B. pearcei, the best mutant rate was in 0.1 g L-1 colchicine medium, and totally 4 polyploidy mutants were obtained. However, both two mutants had the different outcomes to the internode length and leaf ratio. The thick of leaves were increase in both experiment, and the leight of leaf petiole were decline. The relative DNA content was confirmwd by flow cytometry which the polyploidization mutants were successfully obtained.
| Identifer | oai:union.ndltd.org:TW/106NCHU5378023 |
| Date | January 2018 |
| Creators | Hung-Cheng Yu, 游宏程 |
| Contributors | 陳彥銘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 88 |
Page generated in 0.0567 seconds