碩士 / 國立成功大學 / 環境工程學系 / 106 / Toxic cyanobacterial blooms are ubiquitous in both freshwater and brackish water throughout the world. Cyanotoxins, microcystins and cylindrospermopsin for example, may cause liver and/or renal diseases, posing a great threat to human. The risks of cyanobacteria to human health have been mainly assessed for the exposure pathway of drinking water. However, in a few recent studies, inhalation of aerosolized cyanotoxins and cyanobacteria is considered to be another pathway for exposure to cyanotoxins. Therefore, it is important to establish an efficient and feasible detection method for aerosolized cyanotoxins, so that a better way to evaluate the risk of inhalation exposure to cyanotoxins can be established.
In this study, the sampling and analytical methods for aerosolized cyanobacteria and cyanotoxins were first developed. The methods were then applied to collect and analyze the cyanobacteria and cyanotoxins laden air in two eutrophic fresh water bodies in Taiwan, namely Tai-Hu Lake, Kinmen County and Hulupi (HLP) Lake, Tainan City. High volume samplers, total suspended particulate (TSP), were used for sampling the aerosols, while quantitative polymerase chain reaction (qPCR) and enzyme linked immunosorbent assay (ELISA) methods were used to quantify four target genes, including Microcystis, Cylindrospermopsis, microcystins and cylindrospermopsins. According to the results from the first sampling, a pre-treatment method was added to lower the detection limit. The results show that, the detection limit of ELISA can be lowered by putting one eighth of the filter paper, glass beads and 500 µl ASM into the tube and bead beating for 10 minutes, while that of qPCR can be lowered by adding a centrifugation process.
The field sampling in HLP lake show that Microcystis and microcystins were detected in the air samples collected near the HLP lake. The detected Microcystis and toxigenic Microcystis concentrations were 27-756 cells/m3 and 10-51 cells/m3, respectively, while the microcystins concentrations were 0.01-0.023 ng/m3 in the air. This study demonstrates the presence of the harmful cyanobacteria and toxins near the studied eutrophic reservoir/lake. The methods developed can be used to collect and analyze airborne cyanobacteria and cyanotoxins near eutrophic lakes/reservoirs, and the results obtained may be used to evaluate the inhalation exposure and risk for cyanotoxins for public.
| Identifer | oai:union.ndltd.org:TW/106NCKU5515007 |
| Date | January 2018 |
| Creators | Hen-ShengWang, 王瀚生 |
| Contributors | Tsair-Fuh Lin, 林財富 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
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