Immobilization of anammox bacteria with polyvinyl alcohol and sodium alginate / 聚乙烯醇和海藻酸鈉包埋厭氧氨氧化菌之研究

碩士 / 國立交通大學 / 環境工程系所 / 106 / With the continuous development of the social economy and the continuous improvement of people’s living standards, the situation of nitrogen pollution in water bodies is becoming more and more serious. As a typical representative of the biological nitrogen removal technology in the new age, Anammox process has attracted attention due to its environmental protection and energy saving features, and it plays an important role in the shortcut of nitrogen removal. However, the doubling time of Anammox bacteria is long, which has become a major obstacle to the application of the process. Immobilized cell is a type of technology that use physical or chemical methods hold cells in a specific space, without affecting their ability to absorb nutrients, and at the same time have a protective effect to maintain high activity. In this study, we used both polyvinyl alcohol (PVA) and sodium alginate (SA) to form composites materials for embedding Anammox bacteria. In the previous batch experiments, the beads were made of PVA mass concentration of 13% and a SA mass concentration of 2% achieved better removal of nitrogen has been selected, and then the beads prepared from this concentration ratio was used as the experimental group. At the same time, a non-embedded control group was set up, long-term experiments were performed in two identical sequencing batch reactors (SBR), and water quality items were analyzed together with scanning electron microscope (SEM) images. The results show that with the increase of time, the pores inside the carrier can be significantly increased from the initial 10 μm to 50-80 μm, while the microorganisms can maintain a certain activity in the embedded beads. The total nitrogen removal efficiency increased continuously with the increase of the nitrogen loading rate, which eventually reached 81 ± 1%, while the control group reached 92 ± 1%. The experimental group did not show obvious superiority. After the long-term experiment was completed, the microbial activity of the two reactors were tested. During the 18 hours experiment, the nitrogen removal effect of the experimental group was 0.18 g TN-removal/d, and the control group was 0.23 g TN-removal/d, the result was also better in the control group than in the experimental group. The physicochemical parameters such as the mass transfer coefficient and specific surface area of the blank carrier were measured, and compared with related literature. It was speculated that the poor mass transfer characteristics may be the main reason for the poor nitrogen removal effect in the experimental group. In the future, the study on the mechanism of cross-linking between the embedded carrier and the Anammox bacteria, as well as the research on the influence of different embedding conditions on the activity of Anammox bacteria, has important practical significance for the practical application of the embedding technology to the Anammox process.

Identiferoai:union.ndltd.org:TW/106NCTU5515012
Date January 2018
CreatorsWei, Yi-Lin, 魏依林
ContributorsLin, Jih-Gaw, 林志高
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format61

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