碩士 / 國立中央大學 / 生醫科學與工程學系 / 106 / The method to reduce carbon emission and remove excessive CO2 in the atmosphere has become an international environmental issue since greenhouse effect has gained increasing attention in the world. In order to effectively remove CO2 from the mixture and simultaneously increase the economic output, a synthetic photobioreactor system, named perfluorinated photobioreactor system (PPBRS) was established in this study. The system is aimed to utilize the excellent CO2 absorption capacity of the PFC to isolate the CO2 from the mixed gas and deliver the absorbed CO2 under an appropriate ratio (v/v) to the photobioreactor for an improved microalgal growth. Furthermore, the O2 generated from the microalgae photosynthesis will be transferred through PFC adsorption and collected in another container. Our data showed that the 2% (v/v) CO2 brought by PFC may provide the highest growth rate of N. oculata compared to the ones with other settings (1 - 12% (v/v) CO2). In comparison to the cells growing with air, the cells with 2% (v/v) CO2 supply for 10 days exhibited 2-, 1.25-, 1.57-, and 1.37-fold increases of cell concentration, amounts of biomass, total lipid, and eicosapentaenoic acid (EPA), respectively. In terms of PPBRS operation for 10 days where a mixture gas of 60% N2 - 40% CO2 was employed as the model mixed gas, the results showed that the PPBRS was able to effectively isolate CO2 through PFC adsorption and maintain the CO2 concentration in the output N2 in 4% for up to 10 days. In comparison to the group with air injection throughout the time course, our data showed that the cells cultured with PPBRS provided 2.64-fold increase of cell density; 2.17-fold increase of total biomass amount; 2.92-fold increase of total lipid amount, and 3.08-fold increase of EPA production. In additional, the oxygen collection rate was about 82.7% based on the numerical evaluation. Taken together, the developed PPBRS may serve as an effective means for simultaneous CO2 separation, O2 collection, and enhanced microalgae/N. oculata productions that is highly applicable for use in the industry.
| Identifer | oai:union.ndltd.org:TW/106NCU05114005 |
| Date | January 2017 |
| Creators | Jen-Hou Sun, 孫仁厚 |
| Contributors | Yu-Hsiang Lee, 李宇翔 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 104 |
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