碩士 / 國立東華大學 / 海洋生物研究所 / 106 / One new quinone compound, 1',2'-epoxide-flexibilisquinone (1), and one known compound sarcophytonone (2), were isolated from a wild-type soft coral Sinularia sp. In addition, a wild-type soft coral Sarcophyton trocheliophorum yield four known cembrane type compounds, (+)-sarcophytoxide (3), 16-hydroxycembra-1,3,7,11-tetraene (4), laevigatol B (5) and sarcophine (6). The structures of compounds 1-6 were established by 1D and 2D NMR spectral data analysis and compare with literature data.
In the cytotoxic activity test, compound 4 exhibited medium inhibitory on K-562 (Human chronic myelogenous leukemia cell), Molt4 (Human acute lymphoblastic leukemia cell) and U937 (Human histiocytic lymphoma cell) for 72 hrs treatment with IC50 9.33 ± 1.74, 5.84 ± 0.76 and 9.75 ± 2.11 µg/mL, respectively. Furthermore, compound 4 also showed weak inhibitory on PC-3 (Human prostate cancer cell) with IC50 12.59 ± 5.67 µg/mL after 72 hrs treatment.
In the anti-inflammatory activity test, preliminary results confirmed that compounds 3 and 6 significantly inhibited the ability of LPS-induced dendritic cells to secrete the inflammatory cytokine IL-12 at a non-cytotoxic dose. Further experiments showed that compound 3 inhibited the expression of CD80 and CD86 in dendritic cells by limited its ability to display antigens and achieved anti-inflammatory effects.
| Identifer | oai:union.ndltd.org:TW/106NDHU5277014 |
| Date | January 2018 |
| Creators | Jyun-Jhih Lin, 林郡值 |
| Contributors | Jui-Hsin Su, 蘇瑞欣 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 133 |
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