碩士 / 國立東華大學 / 海洋生物研究所 / 106 / There are many sources of marine natural products, including corals, sponges, tunicates, mollusks, algae, and microorganisms. The biological activity of these marine lives can be used as a source of drug development. It is considered as an important pillar for the treatment of diseases, but more and more research shows that many of the active substances present in marine organisms are in fact produced by chemical interactions between symbiotic microorganisms such us bacteria, fungi, algae, etc. The secondary metabolites of marine actinomycetes are considered to be the most important contribution to drug development. This article screened the marine actinomycetes Streptomyces sp., by using ethyl acetate to extract the secondary metabolite, Lu01-M. Lu01-M has anti-prostate cancer effect and the most sensitive cancer cell line was PC3, with IC50 2.45±0.27 μg/mL after 24 hours treatment. Thus, PC3 cells were subjected to further investigation. Lu01-M also affecting cell cycle, inhibited cells proliferation and migration. The use of increasing doses of Lu01-M (0 to 6.25 μg/mL) increased the percentage of disruption of mitochondrial membrane potential, induced the reactive oxygen species and endoplasmic reticulum stress, and inhibited the p-Akt signaling, leading to decrease the cell viability. We further expanded our investigation to evaluate the antitumor effect of Lu01-M in vivo xenograft animal models. Animal experiments showed that Lu01-M could inhibit tumor growth without affecting the body weight of mice and biochemical index. Taken together, these findings suggest that Lu01-M has a great potential in the development of anti-cancer drugs.
| Identifer | oai:union.ndltd.org:TW/106NDHU5277015 |
| Date | January 2018 |
| Creators | Yong-Shiou Lin, 林詠琇 |
| Contributors | Mei-Chin Lu, 呂美津 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
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