碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 106 / Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer death worldwide. In recent years, many bioactive ingredients have been identified from corals and have been demonstrated to possess various bioactivities in vitro, such as anti-inflammatory activity, antibacterial activity, and cytotoxicity towards cancer cells. The objective of this study was to investigate whether the coral extract of Briareum had anti-cancer effect on CRC in vitro and in vivo. The results showed the coral extract dose-dependently decreased the cell viability and induced apoptosis in COLO205 cells which evidenced with an increase of sub-G1 cells by the flow cytometer analysis. Coral extract treatment also induced caspases activation and regulation of protein expression involved in the death receptor and mitochontrial pathway, thus triggered cell apoptosis. In addition, we found COLO205 cells treated with the coral extract caused the increase of reactive oxygen species and mitochondrial dysfunction. COLO205 cells pretreated with antioxidant N-acetylcysteine (NAC) decreased the cytotoxicity caused by coral extract. In COLO205 xenograft model, coral extract (10、50 mg/kg) treatment resulted in suppression of tumor growth by inducing apoptosis, autophagy and inhibiting angiogenesis without causing any side effects. The results of immunofluorescence staining showed that the coral extract effectively reduced the marker of cancer stem cells in tumor tissues. Based on the above results, we suggested the potential of coral extract from the genus Briareum on treating human colorectal cancer.
| Identifer | oai:union.ndltd.org:TW/106NKIM0084007 |
| Date | January 2018 |
| Creators | LI, YOU, 李優 |
| Contributors | LAI, CHING SHU, 賴慶紓 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 118 |
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