碩士 / 國立屏東科技大學 / 動物疫苗科技研究所 / 106 / Edwardsiella tarda has been recognized as one of the important bacterial pathogens to the cultured freshwater and marine fish worldwide. Infections with this bacterium have resulted in major economic losses in Taiwan aquaculture industry. Infection with E. tarda often leads to the development of a systematic disease called edwardsiellosis, characterized by symptoms of anal swelling, hemorrhagic, exophthalmia, liver and kidney severe lesions of internal organs. Since E. tarda is an intracellular pathogen, most antibiotics are not effective to treat edwardsiellosis. Although development of immunizing agents has being studied, effective products are not currently available for use in fish protection from edwardsiellosis. Recent decades, biodegradable and biocompatible alginate gels have been used as safe, potent adjuvants or delivery systems to encapsulate antigens for preparing controlled-release microparticle vaccines. In the study, therefore, sodium alginate was used as an encapsulation material to entrap inactivated E. tarda for producing a microparticle vaccine against edwardsiellosis in fish; The particles were prepared through an emulsion-based methodology employing by homogenizing vegetable oils containing different surfactant Span 80, adjusting the ratios of sodium alginate and oil phase at different homogenization speed and time. The sodium alginate microparticles could be prepared by utilizing 1.5 % sodium alginate and 0.05 % Span 80 at 500 rpm of homogenization speed. These parameters have been appropriate for spherical morphology, particle size distribution and antigen release effect. Based on the in vitro release test, the release efficiency of microparticles could reach up to 80%.
| Identifer | oai:union.ndltd.org:TW/106NPUS5290004 |
| Date | January 2018 |
| Creators | Liu, Shih-Hua, 劉蒔樺 |
| Contributors | Yang, Chung-Da, 楊忠達 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 59 |
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