碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 106 / Large pool of study in marine microbial community has shown that the microbial composition within the community changes with environmental change. Submarine Mud Volcano, with methane, carbon dioxide, and nitrogen constantly erupted into the seawater, represents a unique and extreme environment for microorganisms to reside. However, it is not fully understood how the microbial composition is different than the rest of marine microbial community. The introduction of Next Generation High-throughput Sequencing advanced the field of metadata analysis in microbial community and since then has been widely used in addition to traditional culture and genotyping methods. In the present study, we examined the composition of microbial community near submarine mud volcano located in southwest Taiwan and compared it with the microbial community outside the volcano in the same region.Using next generation high-throughput sequencing, we compared samples collected from sediment, upper-level, mid-level, and bottom-level seawater. The results show the dominant strains are Alphaproteobacteria and Gammaproteobacteria in these sampling points of the surface and the middle seawater, but the bottom of the dominant strains is OD1_ABY1 in mud volcanic region. Also, the dominant strains is Alphaproteobacteria in general seabed region. In addition, the OD1_ABY1 is a bacterium that grow in an anaerobic environment. The methane from the volcanic eruption of the seafloor may cause the environment of some microorganism to be unsuitable. Moreover, the dominant strains of two points of the sediment sample are not the same, and the number of species is far from the same.In conclusion, the microbial composition from the submarine mud volcano is drastically different from the non-volcano area from the same region. The unique environment of submarine mud volcano manipulates the composition of microbial community which causes the difference in dominant species at sediment and bottom-level seawater but not mid- or upper-level seawater. Lack of oxygen along with the increased methane, carbon dioxide, and nitrogen may explain this drastic change.
| Identifer | oai:union.ndltd.org:TW/106NSYS5277004 |
| Date | January 2018 |
| Creators | Yu-Ching Cheng, 鄭宇晴 |
| Contributors | Shu-Fen Chiou, 邱素芬 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 118 |
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