博士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 106 / In this study, the biological activity of aquaculture red algae (Sarcodia suieae). Earlier studies indicated that it has antioxidant and glucose-regulating activity. First of all, four extraction layers (DEE, DEH, DHE, DHH) were selected for anti-inflammation, whitening, bone formation promotion and cytotoxicity screening. It was found that DEE and DEH significantly inhibited the production of inducible nitric oxide synthase (iNOS) by inflammatory proteins produced by macrophage RAW 264.7 stimulated by lipopolysaccharide (LPS), DEH and DHH can promote bone formation and whitening effect, DEE, DEH, DHH, DHE inhibit human prostate cancer cell lines, DEE, DHE, DHH inhibit the effect of human hepatoma cell lines, Then, three extraction layers PD1, PD2 and PD3 were extracted from the alcohol to screen out the anti-inflammatory, bone-forming, whitening, cytotoxic and neuroprotective activities, PD1 could significantly inhibit lipopolysaccharide stimulated inflammatory proteins produced by rat macrophages to inhibit the formation of inducible nitric oxide synthase, PD1 is neuroprotective in both SH-SY5Y human neuroblastoma and zebrafish Parkinson''s mode in neuroprotection tests, PD1, PD2 and PD3 have inhibitory effects on brain cancer cells. PD1 and PD3 can inhibit the growth of hepatoma cells and normal liver cells. PD1, PD2 and PD3 also have the activity of whitening. In addition, the oral administration of PD1 to rats significantly inhibits the carrageenan-induced swelling of the paw of the plantar palpitations and induces inflammatory pain. These include mechanical tactile sensations, thermal hyperalgesia. Histopathology also observed that combined administration of PD1 inhibited the increase of leukocyte infiltration, proinflammatory cytokines and peroxidation caused by carrageenan. The results of this study suggest that PD1 may have the potential to develop anti-inflammatory and analgesic drugs. The results of this study are expected to help scientists continue to develop other biomedical applications of red algae and help to enhance the economic value of red algae.
| Identifer | oai:union.ndltd.org:TW/106NSYS5277005 |
| Date | January 2018 |
| Creators | Chieh-Chih Shih, 石界智 |
| Contributors | Zhi-Hong Wen, 溫志宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 125 |
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