The effects of dietary supplement of grape extract on growth performance and lipid peroxidation of white shrimp, Litopenaeus vannamei / 飼料中添加葡萄萃取物對白蝦成長及脂質過氧化之影響

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 106 / The study was conducted to investigate the effects of dietary supplement of grape extract on the growth performance and lipid peroxidation of white shrimp, Litopenaeus vannamei.
  The effects of grape (Nor-grape 80), polyphenol-rich feed additive, on the growth performance, immune parameters and lipid peroxidation of 0.02 g white shrimp Litopenaeus vannamei were investigated in experiment I and II. Four experimental diets included 50 (N50), 100 (N100), 150 (N150) and 200 (N200) ppm grape extract and a control diet (added 0.1% vitamin E and without dry grape extract) for experiment I, and diets included 250 (N250), 500 (N500), 750 (N750) and 1000 (N1000) ppm dry grape extract and control diet for experiment II were formulated. Each diet was randomly assigned to three replicate groups of shrimp larvae. In experiment I, 0.02g shrimp larvae fed N50, N100, N150, N200 diets had no significantly different in weight gain percentage for 8 weeks. However, shrimp larvae fed N150 and N200 diets had significantly higher weight gain percentage than those fed control diet. In experiment II, 0.02g shrimp larvae fed N250 and N500 diets had significantly higher weight gain percentage than those fed other treatment diets for 6 weeks. The shrimp larvae fed control diet had the lowest weight gain percentage. Survival of shrimp fed treatment diets in experiment I and II were higher than 90%. In experiment I, the superoxide dismutase (SOD) of shrimp fed control and N200 diets was significantly higher than that of shrimp fed other treatment diets. SOD of shrimp fed N50, N100 and N150 diets showed no significant differences. In experiment II, SOD of shrimp fed N250 diet was significantly higher than that of shrimp fed N750, N1000 and control diet. SOD of shrimp fed N250 and N500 diets showed no significant differences. In experiment I, the thiobarbituric acid substances (TBARS) of shrimp fed N50 diet was significantly higher than other treatment diets, followed by N100, N150, N200 and control diet. In experiment II, the shrimp larvae fed N750 and N1000 diets had significantly higher TBARS than those fed other treatment diets. The shrimp fed N250 diet showed the lowest TBARS.
  The effects of Nor-grape 80, polyphenol-rich feed additive from grape, on the growth performance, immune parameters and lipid peroxidation of 2.456 g white shrimp Litopenaeus vannamei were investigated in experiment III. Four experimental diets included 125 (N125), 250 (N250), 375 (N375) and 500 (N500) ppm dry grape extract and a control diet (added 0.1% vitamin E and without dry grape extract) were formulated. Each diet was randomly assigned to three replicate groups of shrimp and cultured for 7 weeks. The shrimp fed N250 had significantly higher weight gain percentage than those fed N125, N500 and control diet. Shrimp fed N250 and N375 had no significantly different in weight gain percentage. The survival of shrimp fed treatment diets for 7 weeks was higher than 90%. SOD of shrimp fed control diet was significantly higher than that of shrimp fed N125 and N250 diets. SOD of shrimp fed N375, N500 and control diets showed no significant differences. The lowest SOD of shrimp fed N125. TBARS of shrimp fed N125 was significantly higher than other treatment diets. The shrimp fed control diet had significantly higher TBARS than that of shrimp fed N250, N375 and control diet. The shrimp fed N500 diet showed the lowest TBARS.

Identiferoai:union.ndltd.org:TW/106NTOU5086012
Date January 2018
CreatorsCheng, Ya-Chi, 鄭雅琪
ContributorsSheen, Shyn-Shin, 沈士新
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format50

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