碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 106 / The objective of this study was to investigate the effects of different combinations of dietary probiotics, including Bacillus subtulis, Micrococcus luteus, and Shewanella sp. on the growth performance and immunity of white shrimp (Litopenaeus vannamei) in the closed culture system, and to evaluate the effect of feed-released probiotics on water quality.
The probiotics were supplemented within diet at a concentration of 2×108 cfu/g; the growth performance, water qualities, total bacteria count and total Vibrio sp. count were measured for 30 days. The results showed that all combination of probiotics significantly promoted the survival rate as compared with control (50%). Among which, groups treated with B. subtulis , M. luteus+Shewanella sp, and B. subtulis+M. luteus had the highest survival rate (92 ± 8% in all three groups). B. subtulis appeared to result in the best growth performance (final weight 15.58 ± 0.06 g), followed by M. luteus (15.11 ± 0.06 g), which were significantly higher than that of the control group (14.22 ± 0.06 g), whereas Shewanella sp. showed no differenes on the growth performance. In terms of the water quality, all treatments showed a significant lowered NH3-N.The group treated with M. luteus only had the lowest concentration (16.95 ± 1.28 ppm), compared with control (40.43 ± 0.87 ppm).Groups treated with B. subtulis only or in combination with M. luteus or Shewanella sp had significantly lowered nitrite-N concentrations (between 28.04- 30.17 ppm), compared with control group (49.04 ppm); All treatments had significantly lowered total Vibrio sp. count (5.39x 102-1.17x103 cfu/mL) as compared with that of control (2.42x103 cfu/mL).
The effects of combinations of probiotics on non-specific immunity in shrimp were evaluated based upon the total blood count (THC), the production ratio of superoxide anion (O2-) and phagocytic activity during 7 days. The results showed that groups treated with M. luteu+Shewanella sp. and B. subtulis had the highest THC in day 4 and day 7 respectively. Group treated with B. subtulis+M. luteus had the highest production of O2- in day 4, while group treated with all probiotics had the highest in day 7. In terms of the phagocytic rate, all treatments showed a significant improvement except for the groups treated with Shewanella sp and M. luteus+Shewanella sp. However, the improvement of phagocytic index was only found in group treated with Shewanella sp. in day 7.
| Identifer | oai:union.ndltd.org:TW/106NTOU5086014 |
| Date | January 2018 |
| Creators | Chiu, Hsu-Cheng, 邱旭晟 |
| Contributors | Nan, Fan-Hua, 冉繁華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 63 |
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