碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 106 / Large quantities of floating brown algae Hizikia fusiformis and Sargassum horneri invade the coast Taiwan from China, Japan and Korea each year. However, these floating invasive algae quickly die decompose shortly after their arrival in Taiwan due to high temperatures. As a result, no natural seedling can be collectedfrom the field in Taiwan. The present study utilized floating H. fusiformis and S. horneri arriving in Taiwan as the source material for tissue culture experiments by induce wounded healing tissues which were used to create algal seeds stock of these species. We found that five-bladed seedlings of H. fusiformis and S. horneri treated with 5μm of uniconazole could be induced to form wounded healing tissues which could redifferrentiate into blade-like seedling and be used as an algal species stock for mass cultivation. Furthermore, S. horneri extracts enhanced growth rates when applied to cultures of H. fusiformis seedlings. Five μm indole-3-acetic acid (IAA), 5 μm 6-benzylaminopurine (BA), and a mixture of 5 μm IAA and 5 μm BA were applied to incised sections of the stipes and haptera of H. fusiformis and the stipes of S. horneri . These treatments resulted in the growth of filamentous tissue at the incision sites in both species was immobilized in sodium alginate beads for the purpose of preserving an algal species stock.
| Identifer | oai:union.ndltd.org:TW/106NTOU5086025 |
| Date | January 2018 |
| Creators | Lu, Yi-Hsuan, 呂怡璇 |
| Contributors | Chen, Yean-Chang, 陳衍昌 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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