碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 106 / The present study investigated the effects of using lactic bacterium Pediococcus acidilactici to control Acute Hepatopancreatic Necrosis Disease in white shrimp caused by Vibrio parahaemolyticus. The lactic bacteria were added in feed and fed white shrimp to test the enhancement of its immunity and growth performance. In addition , the inhibition of V. parahaemolyticus by using the lactic bacteria was also examined. The duration of feeding experiments was two months , and the body length and weight of shrimp were recorded in day 14, 30 and 60, respectively. Aliquots of RNA in hepatopancreas and muscle of three healthy shrimp were extracted for future use .
The results revealed that the feed added with lactic bacteria could enhance growth performance of shrimp at 14 , 30 and 60 days, respectively, compared to the control group . In addition , the lactic bacteria could maintain at stable amount around 105 CFU/ml in shrimp intestine after feeding for four days. For inhibition tests, the lactic bacterium could inhibit V. parahaemolyticus in seawater with a reduction from 105 CFU/ml to about 102 CFU/ml . However , the inhibition in shrimp intestine was less apparent comparing to that of sea water with a reduction from 104 to about 103 CFU/ml.
For the tests of enhancement on shrimp immunity after feeding the lactic bacteria, the changes of LD50 were examined by lethal challenges with V. parahaemolyticus via immeraion or injection. The results showed that at day 14,30 and 60, all the LD50 were apparently higher than that of control group via both challenge routes. In particular, the increase of LD50 could reach to 100 folds at day 60 via immersion route comparing to that of control group. The lysozyme activities in shrimp muscle were not affected significantly after feeding the lactic bacteria. However, there were significant differences of lysozyme activities obtained in shrimp hepatopancreas after feeding with the lactic bacteria at day 14, 30 and 60, respectively, comparing to that of the control group. For the tests of pro-PO gene expression, there were significant differences obtained in shrimp hepatopancreas, but not in muscle comparing to those of the control group.The present study revealed that the use of lactic bacteria P. acidilactici as probiotics could enhance growth performance and immunity of shrimp against vibrio challenge after long-term feeding in white shrimp. In addition, it seemed that the presence of P. acidilactici in sea water or shrimp intestine could inhibit the growth of V. parahaemolyticus.
| Identifer | oai:union.ndltd.org:TW/106NTOU5086030 |
| Date | January 2018 |
| Creators | Jhang, Dun-Hong, 張敦鴻 |
| Contributors | Lee, Kuo-Kau, Liu, Ping-Chung, 李國誥, 劉秉忠 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 51 |
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