碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 106 / TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) is a iipophilic potent environmental toxicant and endocrine-disrupting chemical (EDC) with known developmental toxicity in humans, and model animal of human, and fish. This study focus on the effect of dioxin on heart development. Exposure the 1hpf zebrafish embryos in different TCDD concentration medium, heart rate decreased since 24 hpf, heart function (stroke volume and cardiac output)decreased since 48 hpf. At 72 hpf and 96 hpf, TCDD-treated embryos exhibited altered looping, with the atria positioned distinctly posterior to the ventricles. Moreover, the ventricles and atria in TCDD-exposed hearts became more compact, accompanied pericardial edema and cardiac volume decreased. TCDD may affect genes involved in myocardial development.
Detecting the gene expression involved in embryonic myocardium development after exposure to dioxin, expression of gata4、gata5、gata6、hand2 are higher than control, but mef2c is lower. The expression of Cardiac protein like amhc、vmhc、cmlc1、cmlc2 are decreased. It is shown that TCDD may cause cardiac development defects by affecting the mef2c gene expression. Detecting the gene expression involved in heart disease like smyd1 and tnnt2a after exposure to dioxin, the expression are higher then control. Exposure to Dioxin during the embryonic period may lead to congenital heart disease. The expression of mef2ca、vmhc、smyd1a have dose dependent on TCDD. These three genes hopefully becoming a relevant marker for the detection of dioxin in the future.
| Identifer | oai:union.ndltd.org:TW/106NTOU5086038 |
| Date | January 2018 |
| Creators | Tseng, Yaun-Shyang, 曾彥翔 |
| Contributors | Lu, Jenn-Kan, 陸振岡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 71 |
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