碩士 / 國立臺灣海洋大學 / 食品科學系 / 106 / Taiwan is abundant in sea sand deposits. The main component of sea sand is silicon dioxide (SiO2), and its use is very wide. Crassostrea gigas is the most popular oyster in Taiwan but its edible part is only about 30% of the total weight, and its shell main component is calcium carbonate (CaCO3). We expected to improve the economic value of sea sand and the full use of oyster shell. the purpose of this study was to mix sea sand and oyster shell powder for converting to calcium silicate (CaSiO3) as the feasibility of bone biomaterials.
At present, the biomedical materials using calcium and phosphorus systems are the most widely used. Because of the good biocompatibility of these substituents and the most similar to human bone components, many synthetic bone substitutes such as calcium phosphate cement have been used in the past few years. However, in recent years, it has been pointed out that silica-containing medical materials can promote the formation of hydroxyapatite in bone-defective parts, and oyster shells can provide a large amount of calcium ions required for bone repair.
Previous studies have shown that calcium silicate materials contain good biocompatibility and bone conduction properties. Therefore, the purpose of this study was to use sea sand and oyster shell mix to convert calcium silicate (CaSiO3) as the feasibility of bone biomaterials via solid-state reaction and hydrothermal reaction methods. The use of sea sand and oyster shells as biomaterials can not only increase the availability of waste materials, but also reduce the cost of biomaterials.
In this study, sea sand and oyster shells were mixed at a molar ratio of 1:1, and they were converted in two ways: solid state reaction and hydrothermal reaction. After a series of analysis of X-ray diffraction, Fourier transmission infrared spectrometry, scanning electron microscopy, energy dispersive X-ray spectrometry, and compositional analysis material identification of converted material, it is understood whether the raw material was successfully converted to calcium silicate material and the difference between the two conversion methods for the material configuration and the optimum condition in synthesizing this material could be obtained. And we used ICP-OES to detect the metal ion content of raw materials and materials to determine whether there have harmful metal ions to the human body. The material soaking solution and the material tablets are prepared for biocompatibility evaluation. After MG-63 cells taken for in vitro cell culture, the growth condition of the osteoblasts is observed. Then the cell cytotoxicity, promoting the bone marrow growth, and the ability of mineralization were examined.
After crystal identification and functional group analysis, the crystal phase and functional groups of the HC-1′ (sea sand and calcinated oyster shells were mixed and then treated with hydrothermal reaction) are the most similarity with calcium silicate. The metal ion content of the material was analyzed. The results showed that the material didn’t have harmful metal element to the human body, and there were traces of metal elements that were beneficial to the human body.
In the MG-63 cell culture, the experiment was divided into two groups, the soaking solution group and material tablets group. The cytotoxicity and alkaline phosphatase activity test showed that the material was not cytotoxic. On the contrary, it promoted the growth of cells and elevated the differentiation of osteoblasts. The result of the mineralization experiment, the cells cultured in soaking solution group and tablets group at the incubation day of 14 and 21 days had significant differences when compared to the control group, indicating that the material promoted cell mineralization. After crystal identification, cytotoxicity, and osteoblast differentiation, and mineralization assay, the synthesized calcium silicate material in this study has potential for repairing bone defects.
| Identifer | oai:union.ndltd.org:TW/106NTOU5253020 |
| Date | January 2018 |
| Creators | Chen, Kai-Jyun, 陳凱軍 |
| Contributors | Hwang, Deng-Fwu, 黃登福 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 70 |
Page generated in 0.0019 seconds