碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 106 / Spermine oxidase (SMO), which catalyzed oxidative catabolism of spermine and also produce reactive oxygen species (ROS). According to previous studies in mice, SMO plays important physiological role in skeletal muscle fiber differentiation and pathological role in brain damage under hypoxia stress. However, the function of SMO and polyamines oxidative catabolism in fish has never been reported. Here, we used molecular genetic approach to clarify the relationship between the expression level of SMO and the hypoxia tolerance in zebrafish.
By using Tol2 transposon system, we successfully produced transgenic zebrafishes, which overexpressed Smo in skeletal muscle or central nervous system (CNS) under the control of MLC2 or HuC promoter, respectively. In the transgenic zebrafish, we could detect the higher expression level of SMO mRNA, protein and also higher concentration of ROS. In MLC2-Smo transgenic fish, which overexpressed smo in the skeletal muscle, the muscle fiber were 7% larger than wild type fish. As for the HuC-Smo transgenic zebrafish, which overexpressed Smo in the nervous system, developed normally, but it was found that the expression level of antioxidant genes, such as sod-1 was higher than that of wild-type. When these zebrafish were in anoxia condition, we found the brain of HuC-Smo transgenic fish displayed higher oxidative level and their mortality were also higher than wild type fish.
Our result indicated that the expression level of SMO in the brain is related to hypoxia tolerance of fish. In the future, these transgenic fish model may be applied to screen the treatments which can help fishes against hypoxia and also help to elucidate the function of polyamine in the development of muscle.
| Identifer | oai:union.ndltd.org:TW/106NTOU5613003 |
| Date | January 2018 |
| Creators | Chen, Yun-Qu, 陳韻曲 |
| Contributors | Lin, Han-Jia, 林翰佳 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 53 |
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