碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 106 / Fucoidan is a complex of polysaccharides containing substantial percentages of L-fucose and sulfate ester groups, mainly derived from brown algae. Fucoidan is demonstrated to inhibit proliferation and metastasis in breast cancer, colorectal cancer, prostate cancer, and lung cancer, showing that the polysaccharide may increase the prognosis survival in cancers. Head and neck squamous cell carcinoma (HNSCC) is one of the world top ten most common cancers with its highest occurrence, highly malignant and metastasis. The epithelia to mesenchymal transition is involved in wound healing, organ fibrosis and the initiation of metastasis in cancer progression. This study aimed to evaluate the efficacy of fucoidan as a chemo-target agents to suppress metastasis in HNSCC cells. Fucoidan was prepared from Sargassum hemiphyllum as well as Laminaria japonica and characterized by HPLC and FTIR. Contents of sulfate and polysaccharide from two brown algae by hot water extraction was observed different ratio, and trypsin might improve the efficient of extraction.. Fucoidan was not found cytotoxic activity in SAS and FaDu cells, whereas viability of OECM1 cells were decreased after 48 h treatment of fucoidan. Cell migration of HNSCC cells was suppressed after fucoidan treatment. The expression of Zeb1 and vimentin was increased by fucoidan, showing that unusual effects were elicited by fucoidan in EMT pathway. After HNSCC cells treated with fucoidan for 0, 3, 6, 12 and 24 hours, phosphorylation of Akt（Ser473）and GSK3β were reduced leading to decrease of β-catenin. Our findings demonstrate that fucoidan may reduce the activation of AKT to promote the activity of GSK3αβ leading to inhibiting cell migration of HNSCC.
| Identifer | oai:union.ndltd.org:TW/106NTOU5613017 |
| Date | January 2018 |
| Creators | Chen, Yu-Chen, 陳祐甄 |
| Contributors | Tang, Shye-Jye, 唐世杰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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