碩士 / 國立臺東大學 / 生命科學系碩士班 / 104 / Alzheimer’s disease, AD is one of the most common degenerative brain disease. Research had be certain amyloid β-peptide, Aβ deposit in brain will cause oxidative stress and inflammation which result in learning ability and memory disorders. The aglycone isoflavones produced by fermented one has higher physiological activity than unfermented one. Due to these advantages in these benefits, our goal is to test whether Lactobacillus paracasei subsp. paracasei NTU 101 fermanted soy-milk will have effects on preventing human from AD or not by conducting experiments on NTU 101-fermentated soy milk and its’ extractions. To do these experiment, we continuously injected Aβ40 through out 28 days to built AD pattern with rats and observe the effect of NTU 101 fermanted soy-milk and soy milk on AD prevention. The Morris water maze test result shows that NTU 101-fermented soy milk (L- has better learning ability. Genistein is the best, followed daidzein and equol comes second and third. Genistein can significantly lower the reading of TBARS in cortex. The test result shows NTU 101 fermented soy-milk has significant improvement. The results of immunohistochemistry staining can realize, the Aβ40 protein deposition Aβ group slices on a significant excess than Vh group. In the result of astrocyte and microglia, it shows that NTU 101 fermented soy-milk decrease TLR-2 and RAGE protein. Form the result above, Aβ40 infusion in brain for 28 days continuouse could cause astrocyte and microglia activator incremental, performance attracted a large number of pro-inflammatory factors and the related factors of AD, which lead to memory learning ability disordered. The ethanol extract of fermented soy milk can reduce astrocyte and microglia-activating factor, pro-inflammatory factors and the related factors of AD to improve memory learning ability. Reason comes from the extract containing a large amount of effective ingredients, the aglycone isoflavones genistein and equol. And the best improving effect comes from the impact of genistein.
| Identifer | oai:union.ndltd.org:TW/106NTTU5105006 |
| Date | January 2016 |
| Creators | Zong-Yang Young, 楊宗諺 |
| Contributors | Chun-Lin Lee, 李俊霖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 150 |
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