碩士 / 國立臺灣大學 / 生化科技學系 / 106 / Silk fibroin produced from silkworms has been widely used as biomaterials owing to its remarkable mechanical strength, extensibility, biocompatibility, and ease of biofunctionalization. In this research, we engineered the silk fibroin as a novel platform capable of capturing various species of pathogens by functionalizing pathogen targeting moiety. Specifically, we first generated the silk material into a porous silk sponge through the lyophilization procedure, yielding enhanced porosity and accessible surface area. The structural stability of our engineered sponge was further improved with ethanol treatment. Second, the sponge was functionalized with engineered apolipoprotein H (ApoH) by forming di-tyrosine crosslinking through the catalyzation of horseradish peroxidase (HRP). ApoH, an immune acute phase human plasma protein, serves as an innate immune defense system against infection and exhibits broad spectrum of pathogen binding ability, including viruses, Gram-negative bacteria and parasites. Finally, the platform was demonstrated to be effective in E. coli and norovirus binding. We envision the device we fabricated, yet to be tuned up, can potentially as a device for rapid isolation and high throughput screening of pathogens. Additionally, the technique could be further harnessed in hemodialysis for reducing the risk of infection in the future.
| Identifer | oai:union.ndltd.org:TW/106NTU05106007 |
| Date | January 2018 |
| Creators | Shang-Ru Wu, 吳尚儒 |
| Contributors | 吳亘承 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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