碩士 / 國立臺灣大學 / 生物科技研究所 / 106 / White spot syndrome virus（WSSV）is a major pathogen of aquacultured shrimp. The infection of WSSV occurs not only in shrimp but also in other marine and freshwater crustaceans, including crabs and crayfishes. WSSV has caused large economic losses and there is still no adequate treatment. Unlike vertebrate immunity which is composed of both innate and adaptive responses, invertebrates rely on multiple innate defense reactions to combat infections. However, probiotics have been used in aquaculture for the function of immune stimulation. In this study, three probiotics were used respectively as feed additive: Lactobacillus reuteri Pg4, Bacillus subtilis AC and B. licheniformis CK, all of which have been proved to increase the immune responses in vitro or in vivo experiments. In immune test, both of phenoloxidase（PO）and superoxide dismutase（SOD）activities in probiotic treated groups were significantly higher than normal feed group. In WSSV challenge test, the two Bacillus strains caused the highest survival rate compared to other groups, and the higher PO and SOD activities were also found in the survival shrimps in B. subtilis group. On the other hand, the results of intestinal microbiota analysis proved that WSSV infection could significantly influence the intestinal microbiota, while the L. reuteri Pg4 and B. subtilis AC could decrease some bacteria genera raised by WSSV infection. In conclusion, B. subtilis is the most effective probiotic among three probiotics to improve the survival rate against WSSV infection.
| Identifer | oai:union.ndltd.org:TW/106NTU05111001 |
| Date | January 2018 |
| Creators | Wen-Sheng Ke, 葛文聖 |
| Contributors | Je-Ruei Liu, 劉嚞睿 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 101 |
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