博士 / 國立臺灣大學 / 海洋研究所 / 106 / Marine bacteria are the microorganisms living in seawater, sediments and marine environments. The phylum Proteobacteria is one of the dominant groups in marine bacteria. To investigate the classification of novel marine bacteria in the coastal waters of Taiwan, bacterial isolates were isolated using several different enrichment culture methods and the bacterial species categories were initially identified using molecular methods. A value of less than 98.7% in 16S rRNA gene sequence similarity that is the criteria for determination was used for molecular identification. After preliminary screening, 9 isolates that could be classified as novel species were selected. These novel strains were all classified to be Proteobacteria; one of them belonged to Alphaproteobacteria and the others belonged to Gammaproteobacteria. In addition, this study also reclassify two species that are in doubt about their taxonomic status.
This study shows that two agarolytic strains, AAM1T and AAT1T, could be classified as representatives of two novel species in a new genus. The strain AIT1T which belongs to Idiomarinaceae in the class Gammaproteobacteria and could be classified as the representative of a novel genus and species. A Gram-negative, heterotrophic, marine bacterium, designated strain TT1T, which belongs to Rhodospirillaceae in the class Alphaproteobacteria. On the basis of the polyphasic taxonomic data from this study, strain TT1T is considered to represent a novel species within a novel genus. Two Gram-negative, heterotrophic, aerobic, marine bacterial strains, designated KT1T and KM1T, are described. They could be classified as representatives of two novel Kangiella species. A bacteria strain designated SPT1T which was isolated from an aged-seawater sample and stored at room temperature for more than 7 years. Polyphasic taxonomic data obtained from this study indicated that strain SPT1T could be classified as a novel Spongiibacter species. Strains DM1 and DM2T which were isolated from lagoon sediments of Dongsha Island, Kaohsiung, Taiwan. The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of novel species and genus for the two strains. In phylogenetic studies, the classification of [Glaciecola] lipolytica and [Aestuariibacter] litoralis was unsuitable, and based on sufficient data, a reclassification of [Glaciecola] lipolytica and [Aestuariibacter] litoralis was made and a new genus is proposed to more appropriately accommodate the recognized species of the genera Glaciecola and Aestuariibacter.
| Identifer | oai:union.ndltd.org:TW/106NTU05279033 |
| Date | January 2018 |
| Creators | Ssu-Po Huang, 黃思博 |
| Contributors | 謝文陽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 99 |
Page generated in 0.0433 seconds