碩士 / 國立臺灣大學 / 醫學工程學研究所 / 106 / Vitiligo is a skin disorder characterized by white spot on appearance; and cellular grafting method is an option for vitiligo treatment. However, the success rate is low due to the administration of treatment in the cell suspension form would reduce the cell survive rate. The aim of our experiment is to establish a nude mice model for chitosan-based melanocytes patch transplant. In our experiment, 8 weeks old female nude mice were applied with methoxsalen for 30mins and exposed to 8J ultraviolet A irradiation, as a recipient sites preparation. This PUVA exposure would induce sunburn blisters within two days, and the blister roof can be peeled off by a waxing patch.
Our control group consist of direct transplant of melanocytes suspension. The result showed that melanocytes successfully attached to the dermis within 24 hours; and epidermis coverage was observed within 72 hours with melanocytes stay at the basal layer. In the experimental group transplant by chitosan membrane, melanocytes spheroids were successfully transplanted onto nude mice. Turns out that human melanocytes can survival on nude mice epidermis and migration into hair follicle.
In the in vivo setting, melanocytes migration were absent; and hence we constructed a in vitro model to study a melanocytes migration through pigmented epidermis equivalent. Melanocytes were fluorescence labelled and the migration of these cells were captured using time lapse camera for 24 hours. The results revealed that melanocytes would actively migrate toward the regions which consisted of keratinocytes and regions of low melanocytes density. Additionally, dark colored melanin were also successfully transferred to surrounding keratinocytes.
In conclusion, the nude mice model were successfully demonstrated as a preclinical model for the melanocyte transplantation based on the chitosan based melanocyte spheroid patch; with the migration process clearly observable in the in vitro model. Potentially, this could lead to a better understanding in the treatment for depigmentation.
| Identifer | oai:union.ndltd.org:TW/106NTU05530047 |
| Date | January 2018 |
| Creators | Yu-Ting, Tseng, 曾妤庭 |
| Contributors | Sung-Jan Lin, 林頌然 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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