碩士 / 國立臺灣科技大學 / 化學工程系 / 106 / Cyanophycin granule polypeptide consists of aspartic acid as a backbone with arginine as the side chain. Compared with the natural cyanophycin, some of the arginine molecules are replaced by lysine when it was produced by genetic recombinant bacteria. The polypeptide possesses an upper critical solution temperature (UCST) type of thermal responsiveness. The morphology can be changed by temperature in the aqueous solution.
We modified the phase transition temperature of insoluble cyanophycin (inCGP) to improve its biological applications by crosslinking with hyaluronic acid (HA), a hydrophilic polysaccharide. Combining the inCGP-HA with gold nanoparticles (AuNPs) can alter its morphology by near-infrared (NIR) radiation because of the photothermal effect of the AuNPs.
Doxorubicin (dox) can be encapsulated in inCGP-HA-AuNPs, and the drug could be released by NIR radiation which increased the local temperature. The drug release was also dependent on the energy density of NIR. Furthermore, the ratio of carrier to Dox and the existence of AuNPs affected the encapsulation efficiency and drug release. Finally, we examined the stability at 4 ℃in phosphate buffered saline (PBS) and 37 ℃in 10 % fetal bovine serum (FBS). In conclusion, the nanoparticles of drug release triggered by NIR radiation showed a potential drug delivery system.
| Identifer | oai:union.ndltd.org:TW/106NTUS5342061 |
| Date | January 2018 |
| Creators | Yu-Chih Lin, 林育慈 |
| Contributors | Wen-Chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 102 |
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