碩士 / 國立臺灣科技大學 / 化學工程系 / 106 / The versatility of a microfluidic reactor was demonstrated in this thesis by applying this technique to synthesize microspheres. In the first part, microfluidic reactor was used to prepare microspheres (MS). The formation of MS were initiated when the oil phase met an aqueous phase in a cross region of the microfluidic reactor. Afterward, MS would become a hydrogel because of the crosslink reaction between sodium alginate and calcium chloride at the interface between oil and aqueous phases, which kept the round shape of microsphere. In the second part, the encapsulation of different types of materials into MS were investigated and the resulting materials were characterized. Finally, in the third part, the encapsulation of four different charges of surfactants to improve the solubility and loading amount of curcumin in the MS were studied for drug release application.
In the first part, MS were prepared by a microfluidic reactor with optimized parameters. The chemical functional group of MS was characterized by Fourier Transform Infrared spectroscopy (FTIR). The results showed that the characteristic peaks of carboxyl and calcium ions existed which indicates that oleic acid was successfully conjugated with calcium chloride. From optical microscopy (OM) image, the amount of fragment decreased and the diameters of MS became smaller and more uniform with increasing the concentration of CaCl2 from 0.1 to 1 w/v%. The diameters of microspheres could be manipulated from 63 to 2314 m when the flow rates of oil and aqueous phase were varied between 0.02 and 0.5 ml/min. Therefore, microfluidic reactor can prepare calcium alginate microspheres with different diameter sizes.
The second part of this thesis is to investigate the encapsulation ability of as-prepared MS. The characteristics of the encapsulation of several materials: (i) Fe3O4, (ii) GFP, (iii) L-929 mouse fibroblast cell, (iv) silver sulfadiazine (AgSD), and (v) curcumin into MS were studied. From the OM image and FTIR spectra analyses, the diameters of MS increased after the encapsulation of as-studied materials and characteristic peaks which belong to the encapsulation are present. From the magnetic tests, the MS loaded with Fe3O4 can be attracted by a magnet. From fluorescent images, the MS loaded with GFP exhibited a green color. The result of the lactate dehydrogenase (LDH) assay showed that the cell number of MS increased from 5000 to 25000 after one day of culture inside the MS. Therefore, the as-prepared MS were able to encapsulate different types of materials.
In the third part of this work, the release properties of curcumin loaded into MS were examined. In order to solve the limitation of curcumin poor solubility in water, four types of surfactants were investigated: Triton X-100、Tween 20、cetyltrimethylammonium chloride (CTAC)、and sodium dodecyl sulfate (SDS). From the morphology analysis by OM, the morphology of MS with Triton x-100, Tween 20, CTAC and SDS varied from microspheres to microspheres, microspheres with tails, and cylinder, respectively. From the FTIR spectra, the characteristic peaks belonging to oleic acid and curcumin existed, showing the successful incorporation of curcumin into the MS. From the X-ray diffraction (XRD) spectra, the crystallinity of curcumin showed an amorphous structure after mixed with surfactants and encapsulated inside the MS. From thermogravimetric analysis (TGA) curves, the difference of residue weight compared MS with MS contain Tween 20 or Triton and curcumin at 500℃ which represented to the loading amounts of curcumin were 1.52 % and 1.08 %, respectively. According to the result, Tween 20 was chosen to study the release profiles of curcumin in PBS buffer. The results of curcumin release showed that MS with Tween 20 were the carriers with adjustable release rate. The drug release mechanism was found to be the simultaneous diffusion and erosion where diffusion was the main mechanism from the mathematical simulation of the Korsmeyer-Peppas and Kopcha models.
| Identifer | oai:union.ndltd.org:TW/106NTUS5342103 |
| Date | January 2018 |
| Creators | Yueh Huang, 黃岳 |
| Contributors | Meng-Jiy Wang, 王孟菊 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 126 |
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