碩士 / 南臺科技大學 / 生物科技系 / 106 / Astaxanthin is a carotenoid which is known as “the king of antioxidant”,
Astaxanthin exhibits high antioxidant activity superior to most of the hydrophobic
antioxidants, showing pharmaceutical and nutraceutical value to be developed in
commercial production, mainly in aquatic plants and animals. When the red cyst
exposed to adverse growth conditions or environmental stress, such as the lack of
nutrient sources (nitrogen or phosphorus), salt is too high or strong light irradiation
will stimulate the green cells begin to turn into red cells and begin accumulation of
secondary metabolites-astaxanthin.
Therefore, this research aims to increase astaxanthin and the biomass of the
microalga by using different combination of illumination on with 95% EtOH extracts ,
we compare the result of different concentration level of antioxidant and survival rate
of normal cells and tumor cells.
The test results of ABTS, DPPH show that the radical scavenging rate of extract,
extracted by 95% EtOH which composed of Haematococcus pluvialis cultured under
5000 Lux, obtained the following performance order from highest to lowest: Blue
group (BA60)> White group (WA60)> Red group (RA60), respectively. The HPLC
indicates that the extracts of Haematococcus pluvialis cultured under three kinds of
LED light obtain the following results, showed in the order of the area of astaxanthin
isoforms from greatest to least: White light group (WA60), Red light group (RA60),
Blue light group (BA60), respectively. Cultured under White light, Blue light and Red
light, the Haematococcus pluvialis extracted by 95% EtOH, with a concentration of
25μg/mL, can induce survival rate in CHO-K1.With 100μg/mL on MDA-MB231, the
survival rate after 24 hours for the three groups WA60, BA60, RA60 displayed the following results: 63%, 62%, 58%, respectively. As a result, the survival rate did not decrease over time.
These results demonstrate that Haematococcus pluvialis cultured under red and
blue light with UV-irradiated can not only shorten the time to generate secondary
metabolites, but also increase the antioxidant properties and antitumor activities.
| Identifer | oai:union.ndltd.org:TW/106STUT0111003 |
| Date | January 2018 |
| Creators | CHOU,YU-HUA, 周侑樺 |
| Contributors | LEE,SHUN-LAI, 李順來 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 69 |
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