碩士 / 義守大學 / 生物科技學系 / 107 / Antrodia camphorata (AC) is a folk medicinal herb used in anti-cancer, hepatoprotective, antitoxic and alcohol-sobering for a long time. Recent studies have discovered that AC extract has pharmacological activity of anti-inflammation, anti-tumor, anti-virus, anti-bacterial functions. The amount of extraction waste from the wood-cultivated AC extraction waste (ACEW) is estimated to be several tones per year in Taiwan, which might cause environmental concerns. Application of ACEW in aquaculture could potentially increase its economic value.
At the beginning of this study, the total amounts of triterpenoids and polysaccharides of ACEW were quantified as 2.91 mg/g and 42.4 mg/g, respectively, for the ultrasonic extracted sample. Next, ACEW was used as feed additive for zebrafish culture. ACEW significantly inhibited the growth of microorganism in the stand-alone tank. For 10% ACEW diet, compared to the control, the total biomass reduced 39.4% at day 5. The feed efficiency of zebrafish with 10% ACEW diet for 30 days was 1.22-fold to that of the control. ACEW improved the tolerances of zebrafish under the heat and cold stresses. When the water temperature reached 38°C or 11°C, respectively, all fish of the control group died; while the 10% ACEW diet group still had 91.7% and 83.3% survival rate, respectively. The wound recovery of zebrafish in half-length caudal fin amputation was promoted from 68.4% to 93% with 10% ACEW diet for 3-week regeneration. There were no significant differences between the 10% ACEW diet group and the control group in tank water pH, fertility and hatching rate. After 30 days of feeding with 10% ACEW and then using oxazolone to induce acute ulcerative colitis, as a prevention model, the expression of colitis-associated cytokines TNF-α and IL-1β can be significantly reduced to 46.7% and 42%, respectively. But for the therapeutic model, i.e. induced colitis first and then fed ACEW, ACEW had no obvious effect on inhibiting inflammation for 1, 3 or 5-day treatment. In summary, feeding ACEW can increase the resistance to dramatic temperature changes, feed utilization, caudal fin regeneration and anti-inflammatory activity of zebrafish. Therefore, the waste extracted from AC fruiting body has the application potential in aquaculture.
| Identifer | oai:union.ndltd.org:TW/107ISU05111001 |
| Date | January 2019 |
| Creators | Wei Wang, 王威 |
| Contributors | Jung-Ren Chen, 陳重仁 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 59 |
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