碩士 / 國立成功大學 / 化學工程學系 / 107 / In this study, two batches of fatty acid mixture cultivated from microalgae- Thraustochytrium sp. DJ3 strain were utilized to prepare drug delivery carriers. We also fabricated vesicle by docosahexaenoic acid (DHA) and compared the results with microalgal lipids. The vesicles had the size between 150 to 200 nm, while the negatively charged character could be explained by the partial dissociation of the fatty acid molecules. The vesicles could be stable up to 700 days and possessed high physical stability. As for the drug encapsulation, we tried to encapsulate hydrophobic substance-lutein and hydrophilic substance-arbutin, respectively. The successful encapsulation of arbutin by microalgal lipid carriers proved the formation of vesicular structure. For the encapsulation of lutein, the initial size increased due to the insertion of lutein molecules into the bilayer structure. The partial protonation of lutein molecules shifted the zeta potential of the vesicles to a less negative value. From both fluorescence polarization and Fourier-transform infrared spectroscopy, the bilayer fluidity decreased after encapsulating lutein, and one would expect less leakage of the vesicular structures. Tracing the variation of encapsulation efficiency through time, it was found that the higher the concentration of lutein encapsulated, the more effective the vesicles could protect lutein from degradation. In hemolysis and cell viability test, all the vesicles could be considered as non-toxic at a concentration lower than approximately 0.25 mM. The results showed that microalgal lipid vesicle was capable of encapsulating both hydrophilic and hydrophobic substances and possessed high biocompatibility. Therefore, vesicles fabricated from microalgal lipids and DHA are potential drug delivery carriers with further applications.
| Identifer | oai:union.ndltd.org:TW/107NCKU5063107 |
| Date | January 2019 |
| Creators | Yu-ChenLin, 林妤臻 |
| Contributors | Chien-Hsiang Chang, 張鑑祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 137 |
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