Comparative FISH mapping of 4 linkage groups betweenPhalaenopsis aphrodite and Phalaenopsis equestris. / 台灣白花蝴蝶蘭與蘭嶼姬蝴蝶蘭的四個連鎖群的比較性螢光原位雜合圖譜

碩士 / 國立成功大學 / 生命科學系 / 107 / With more than 28000 species, Orchidaceae is the biggest family in angiosperms. Phalaenopsis becomes one of the most popular groups of orchids because of their various morphology and colors. In Taiwan, there are two native Phalaenopsis species, P. aphrodite and P. equestris. Both of them are served as important parents in orchid breeding programs. Therefore, understanding and revealing the genetic information of the two species by cytogenetic analysis will be beneficial to orchid breeding. Here, we selected four linkage groups of P. aphrodite and systematically identify their positions on pachytene chromosomes of P. equestris by comparative fluorescence in situ hybridization (FISH) mapping. Our results showed that the four linkage groups are conserved and there is collinearity between some chromosomes of P. aphrodite and their corresponding chromosomes in P. equestris. We also identified chromosomal rearrangements between the two species. Overall, our results revealed the precise positions of the four linkage groups on a single chromosome in P. equestris which will facilitate the identification of each chromosome. The results gained in this study will contribute to systematic orchid breeding and enhance the understanding of orchid evolution.

Identiferoai:union.ndltd.org:TW/107NCKU5105032
Date January 2019
CreatorsShang-RenChang, 張尚仁
ContributorsSong-Bin Chang, 張松彬
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languageen_US
Detected LanguageEnglish
Type學位論文 ; thesis
Format73

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