碩士 / 國立東華大學 / 海洋生物研究所 / 107 / As the oceans were continuously threatened by anthropogenic pollutions and climate change, coral breeding has become the preferred conservation method to prevent exposure to suboptimal environment conditions which could be detrimental to their rehabilitation. However, it is also a concern that sexual reproduction capability of the ex-situ coral would be affected in the artificial environment. The aim of the study was to observe and compare the ultrastructure of the wild Oxypora lacera and E. gemmacea oocytes and their respective in-vivo cultured counterpart using transmission electron microscope (TEM). TEM analyses of O. lacera showed oocytes collected from the wild were larger (389.32 ± 21.32 µM) than the cultured oocytes (302 ± 13.75) but the microvilli of the wild O. lacera oocytes were shorter (2.21 ± 0.21 µM) than those found on cultured oocytes (3.02 ± 0.68 µM). Similar differences were also found observed in the E. gemmacea whereby oocyte from the wild were larger (268.91 ± 10.02 µM) than the cultured (207.25 ± 16.72 µM) but the microvilli in the wild is shorter (1.18 ± 0.22 µM) than the cultured oocytes (1.93 ± 0.14 µM). Internally, yolk body and lipid granules in the wild oocytes of O. lacera (31.87 ± 6.92 and 16.64 ± 3.81 µM respectively) and E. gemmacea (1.16 ± 0.31 and 7.21 ± 0.89 µM respectively) were smaller than the yolk body and lipid granules of cultured oocytes O. lacera (33.99 ± 3.10 µM and 23.05 ± 3.02 µM respectively) and E. gemmacea (1.28 ± 0.61 and 8.88 ± 0.51 µM respectively). In addition, yolk materials abundance in the both species oocyte were also lower in the wild (O. lacera - 55.4 %; E. gemmacea - 50.1%) compared to the cultured oocytes (O. lacera - 81.2 %; E. gemmacea - 74.5%). Although it is evident that culture environment is the main factor for these differences, the actual reason for these changes were still unknown. However, it was suggested that nutrient availability played a significant role in determining formation of lipid body and yolk granules. The present ultrastructure study would facilitate future works on coral breeding for conservation purpose.
| Identifer | oai:union.ndltd.org:TW/107NDHU5277003 |
| Date | January 2019 |
| Creators | Jian-Ming Zhuo, 卓建銘 |
| Contributors | Chiah-Sin Lin, 林家興 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 52 |
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