碩士 / 國立東華大學 / 海洋生物研究所 / 107 / In this study, we used the mitochondrial COI gene to examine the phylogenetic and population genetic structure of the genus ,Neocaridina,27 site in the 9 river systems and Kinmen and Xiamen regions of Fujian,from China, in addition, according to the river system and regional groups, it is divided into Jiulong River, Mulan River, Hanjiang River, Minjiang River, Jinjiang River, Dongxi River, Pearl River, Yangtze River, Huanggang River, Jinmen and Xiamen. A total of 334 samples were used and there were 48 haplotypes. In the results of the phylogenetic analysis, there were five species, Neocaridina palmata、Neocaridina davidi、Neocaridina ketagalan、Neocaridina heteropoda,Neocaridina sp. was not confirmed, respectively. The main population is N.palmata,so we used 197 samples of N.palmata.There are 30 haplotypes,Haplotype diversity = 0.825,Nucleotide diversity = 0.00395, Fu’s Fs =–15.725***,Tajima’s D =–1.54469,High level of Hd,low level of Nd and neutral test were negative,which means that populations had expanded,this result also be supported by Mismatch distribution analysis. Pairwise FST estimates show that the SSS at Dongxi is extremely high differentiated from all other river systems, but the differentiation of some ethnic populations in other river systems is relatively low, indicates that N.palmata can communicate across river system barriers.In the AMOVA analysis,there no obvious genetic differences among groups and among population within groups , and the variation was mainly within populations. There is no obvious grouping between the haplotypes of the Minimum spanning network. We used 74 samples of N.davidi.There are 8 haplotypes,Haplotype diversity = 0.670,Nucleotide diversity = 0.00437, Fu’s Fs =1.580,Tajima’s D =–0.19360. The haplotypes of the Minimum spanning network are geographically separated by several geographical branches, the island group (Kimmen Island KM and Xiamen Island WSL), the coastal group (Jinjiang AX, HM and Mulan River MU, LH), the inland group (Minjiang River SC, WY and Yangtze River SK), respectively.Because N.davidi is a common species in the aquarium market, and N.davidi is not continuously distributed, it is speculated to be caused by external migration.
Identifer | oai:union.ndltd.org:TW/107NDHU5277011 |
Date | January 2019 |
Creators | Jin-Jia Zhang, 張晉嘉 |
Contributors | Chiao-Chun Han, 韓僑權 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | zh-TW |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 70 |
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