碩士 / 國防醫學院 / 公共衛生學研究所 / 107 / Background：
Welding metal fumes are a complex mixture contains particulate and metals that workers could expose through inhalation and ingestion routes. Welding fume particulate matter and its components drive endogenous formation Advanced glycation end-products (AGEs) has not been explored.
Objectives：
Urinary and toenail metals are measured as short-term and long-term exposure biomarkers, while blood AGEs and skin AGEs are measured as short-term and long-term biological effect markers. Specific aims include:
1. To explore the relation between short-term (urinary metals) or long-term (toenail metals) biomarker of exposure and plasma Advanced Glycation End Products concentrations.
2. To explore the relation between short-term (urinary metals) or long-term (toenail metals) biomarker of exposure and skin Advanced Glycation End Products indicator.
Methods：
We conducted a cross-sectional study. Study participants consisted of 73 welders and 27 office workers. Exposure assessment was based on eight-hour measurement with a personal PM2.5 sampler of exposure to metal fume fine particle matter on Monday. On Tuesday morning, participants were instructed to fast for at least 10 hours prior to blood collection, urine collection, and collect toenail collection, Skin autofluorescence (SAF). Skin autofluorescence (SAF) was measured by a AGEs reader. Plasma AGEs was analyzed by ELISA kits. Urinary metal concentration was analyzed by ICP- MS. SPSS 22.0 was used to perform statistical analysis.
Result：
PM2.5 concentrations were significantly higher in exposed group (555.64±472.08 μg/m3) compared to that of control group (258.02±279.21 μg/m3)(p=0.001). Concentrations of urinary chromium, urinary manganese, urinary iron, urinary copper and urinary lead were higher in exposed group compared to control group. In addition, concentration of toenail manganese and toenail iron were higher in exposed group than that of control group. There were a positive association between urinary manganese (β=0.279, p=0.006), urinary iron (β=0.273, p=0.007) and urinary lead (β=0.290, p=0.005) with plasma AGEs after adjusted creatinine and smoking status, respectively. Furthermore, urinary vanadium (β=-0.203, p=0.049), and urinary selenium (β=-0.207, p=0.041) were statistically significant negative associated with skin AGEs indicator after adjusted creatinine and smoking status. toenail zinc (β=-0.209, p=0.022) was negatively associated with plasma AGEs after adjusted smoking status and work type.
Toenail copper (β=0.243, p=0.017), and toenail mercury (β=0.298, p=0.003) were positive associated with skin AGEs indicator after adjusted confounders, respectively.
of Exposure group and control group have significant differences of urinary cadmium and toenail selenium. But toenail cobalt (β=-0.244, p=0.018) was negative associated with skin AGEs indicator after adjusted confounders.
Conclusion：
1. Concentration of urinary manganese, urinary iron and urinary lead are associated with increased AGEs concentration.
2. Concentration of urinary vanadium and urinary selenium are associated with decreased skin AGEs indicator.
3. Toenail zinc is associated with decreased plasma AGEs level.
4. Toenail copper and toenail mercury are associated with increased skin AGEs indicator.
5. Toenail cobalt is associated with decreased skin AGEs indicator.
| Identifer | oai:union.ndltd.org:TW/107NDMC0058036 |
| Date | January 2019 |
| Creators | SHEN, KUAN-YU, 沈冠宇 |
| Contributors | Lai, Ching-Huang, 賴錦皇 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 152 |
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