碩士 / 國立高雄科技大學 / 水產食品科學系 / 107 / Mercury is a common heavy metal pollutant. Among them, methylmercury, a metabolite formed by microbial metabolic conversion, is the most toxic, 10 to 100 times more toxic than general inorganic mercury. The purpose of this study was to establish a simple, practical, and fast method for the determination of methylmercury in fish and shellfish. This liquid chromatography coupled to inductively coupled plasma mass spectrometry (LC-ICP-MS) was used to determine mercury species including the separation and quantification of methylmercury (Me-Hg), propylmercury (Pr-Hg) and divalent mercury (Hg2+). The chromatographic column, mobile phase pH, ICP-MS plasma power in-tensity and aerosol gas flow rate, and sample extraction solution condi-tions are optimized. Moreover, the LC-ICP-MS was established to de-termine the validity of the fish samples, and the mercury species in the edible meat were analyzed by the established method for the fish sold in southern Taiwan.
The results showed that the mixed solution of 50 mM L-cysteine and 50 mM sodium perchlorate (pH=4.0) used as the mobile phase, and IonPacTM CS5A (4×250 mm, 9.0 μm) column with ion exchange resin for LC method are the optimum conditon. In ICP-MS condition, the plasma power set at 1550 W and the carrier flow rate of 0.96 L/min are the op-timum conditions for analysis. In addition, 0.04, 0.1 and 0.2 mg/kg concentrations of Me-Hg, Pr-Hg and Hg2+ standards were spiked to fish samples, and the average recovery (accuracy), repeatability (CV%) and intermediate precision (CV%) obtained by LC-ICP-MS ranged from 86.13 to 101.37%, 1.45 to 3.69% and 1.85 to 5.01%, respectively, all in accordance with the validation of food chemical testing methods. Moreover, the analysis of the fish sold in southern Taiwan by the es-tablished method showed that the methylmercury contents in tuna and marlin fish meats, and salmon and cobia fish meats all were lesser than the methylmercury limit of food hygienic standard (2 mg/kg and 0.5 mg/kg, respectively).
| Identifer | oai:union.ndltd.org:TW/107NKUS0084007 |
| Date | January 2019 |
| Creators | Hsu, Chu-Jung, 許祝榮 |
| Contributors | , 蔡永祥, 李憶甄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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