碩士 / 國立高雄科技大學 / 環境與安全衛生工程系 / 107 / In this study, ammonia nitrogen was used as a substrate, and a hydrogen-producing microbiome(HMB) was added to provide a bacteriological source-derived algae-bacteria symbiosis system. The experiment was divided into two stages.In the first stage, batch experiments were carried out with 7 ammonia nitrogen concentrations to investigate the effects of ammonia nitrogen on photoreaction and dark reaction units and the optimal initial domestication concentration.The second stage is a continuous algae column experiment, which is divided into two units of photoreaction and dark reaction. The algae-bacteria symbiosis system is domesticated at the optimal starting concentration known in the first stage, and the ammonia nitrogen concentration is increased with time to explore the symbiotic relationship between ammonia and nitrogen. The influence of the system, and the chlorophyll a and the total number of colonies, the spectrophotometer wavelengths of 600 nm and 640 nm can be used to understand the feasibility of the concentration of bacteria and algae.
The results of the first stage experiment showed that the symbiotic system of algae was the best with 50 mg/L NH4+-N, the removal rate of photoreaction ammonia nitrogen was 99%, the dark reaction was 76%, and the algae concentration increased obviously. The concentration is low due to insufficient algae concentration, which leads to excessive ammonia concentration and inhibits growth. Although the removal rate is 75%, the algae concentration decreases with time. Therefore, the second stage experiment is started with ammonia nitrogen concentration of 50 mg/L NH4+-N. After domestication, the ammonia nitrogen concentration was increased in sequence and the ammonia nitrogen removal rate was over 75%. The maximum volumetric load of the system was calculated to be 0.3 mg NH4+-N/MLVSS day. The chlorophyll a and the total number of colonies were compared with the data of the spectrophotometer. The results showed that the total number of colonies was different from the growth trend measured at a wavelength of 600 nm. The concentration of the system was not known at a wavelength of 600 nm; chlorophyll a and the growth trend measured at a wavelength of 640 nm is different, so the wavelength of 640 nm is known to the concentration of algae in the system. The reason is that the bacteria are less in the system than the algae in the system, so it is impossible to split the light. The photometer measures the concentration of the bacteria, whereas the majority of the system is algae, the concentration of algae can be measured by this method.
| Identifer | oai:union.ndltd.org:TW/107NKUS0519039 |
| Date | January 2019 |
| Creators | SHEN,YU-KAI, 沈育楷 |
| Contributors | LAY,JIUNN-JYI, 賴俊吉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 83 |
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