碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 107 / Effective bone regeneration strategy and therapy are crucial in view of the aging population trend worldwide. Osteogenesis plays a critical role in bone regeneration. In this study, we aim to identify an osteogenesis-promoting compound using the MC3T3-E1, murine calvarial pre-osteoblast cell line. We then examined osteogenic effects in vivo on a zebrafish development model and rat calvarial defect model. PD-1, the ethyl-acetate extract from Sarcodia ceylanica, upregulated alkaline phosphatase (ALP) activity in MC3T3-E1, whereas OA-4, a pure compound isolated from PD-1, not only similarly enhanced ALP activity, but also exhibited osteogenesis-promoting effects through activation of P38 and extracellular signal-regulated kinases (ERK) signals. OA-4 promoted osteogenic differentiation, and increased the mineralization, as well as exhibiting osteogenesis-promoting effects in vivo. First, 100 M of OA-4 increased the vertebrae calcification on zebrafish larvae. Second, rats with calvarial bone defect subcutaneously injected with OA-4 (1 and 5 mg/kg/day for 28 d) exhibited improved recovery and OA-4 was found through histological analysis to enhance osteogenic protein expression. Our findings indicated that OA-4 is an osteogenesis-promoting compound, and that further exploration of its application of OA-4 in fracture therapy is warranted.
| Identifer | oai:union.ndltd.org:TW/107NSYS5277007 |
| Date | January 2019 |
| Creators | Yi-Wei Luo, 羅翊維 |
| Contributors | Zhi-Hong Wen, 溫志宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 59 |
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